PDF(Pubmed)
Abstract：
OBJECTIVE: Recent studies have described a significant role for neutrophils in reproductive processes and their participation in the preparation of the cervix for childbirth and the activation of labor, in the postpartum involution of the uterus, and in the occurrence of preeclampsia. This study aimed to assess the formation of free radicals by neutrophils in the blood of women on the first day after childbirth and to characterize the adrenergic effect on this process.
METHODS: Venous blood samples from 100 female volunteers aged 26-32 years who had 2 or 3 full-term deliveries were collected and analyzed. Various adrenergic compounds were considered (agonists alphaand betaadrenoreceptors, adrenoblockers). The intensity of the respiratory burst of neutrophils and the effect of adrenergic substances on them were assessed with latex-induced luminol-dependent chemiluminescence.
RESULTS: Neutrophil activity depends on the stage of the woman\'s reproductive process: it decreases during pregnancy, reaches the lowest values during childbirth, and increases significantly in the first hours after childbirth. On the first day after childbirth, alpha-1-adrenergic receptors are highly active in neutrophils, through which NADP-H-oxidase is activated and activated oxygen species are formed. At the same time, alphaor beta-agonists inhibit the radical activity of cells.
CONCLUSIONS: Latex-induced oxidative burst of female blood neutrophils correlates with the stage of the reproductive process. Stressful conditions in the postpartum period can suppress the ability of neutrophils to release reactive oxygen species, which increases the risk of postpartum infections.
METHODS: Venous blood samples from 100 female volunteers aged 26-32 years who had 2 or 3 full-term deliveries were collected and analyzed. Various adrenergic compounds were considered (agonists alphaand betaadrenoreceptors, adrenoblockers). The intensity of the respiratory burst of neutrophils and the effect of adrenergic substances on them were assessed with latex-induced luminol-dependent chemiluminescence.
RESULTS: Neutrophil activity depends on the stage of the woman\'s reproductive process: it decreases during pregnancy, reaches the lowest values during childbirth, and increases significantly in the first hours after childbirth. On the first day after childbirth, alpha-1-adrenergic receptors are highly active in neutrophils, through which NADP-H-oxidase is activated and activated oxygen species are formed. At the same time, alphaor beta-agonists inhibit the radical activity of cells.
CONCLUSIONS: Latex-induced oxidative burst of female blood neutrophils correlates with the stage of the reproductive process. Stressful conditions in the postpartum period can suppress the ability of neutrophils to release reactive oxygen species, which increases the risk of postpartum infections.
摘要：
目的：最近的研究描述了中性粒细胞在生殖过程中的重要作用，以及它们在准备分娩子宫颈和激活分娩中的参与，在子宫的产后复旧，以及先兆子痫的发生。这项研究旨在评估分娩后第一天妇女血液中中性粒细胞形成的自由基，并表征肾上腺素对这一过程的影响。
方法：收集并分析了100名年龄在26-32岁、有2或3个足月分娩的女性志愿者的静脉血样本。考虑了各种肾上腺素能化合物（激动剂α和β肾上腺素受体，肾上腺素受体阻滞剂）。用乳胶诱导的鲁米诺依赖性化学发光评估中性粒细胞呼吸爆发的强度和肾上腺素能物质对它们的影响。
结果：中性粒细胞活性取决于女性生殖过程的阶段：它在怀孕期间减少，在分娩时达到最低值，并在分娩后的头几个小时显着增加。分娩后的第一天，α-1-肾上腺素能受体在嗜中性粒细胞中高度活跃，NADP-H-氧化酶通过其被活化并形成活化的氧物种。同时,α或β-激动剂抑制细胞的自由基活性。
结论：乳胶诱导的女性血液中性粒细胞的氧化爆发与生殖过程的阶段相关。产后期间的压力状况可以抑制中性粒细胞释放活性氧的能力，这增加了产后感染的风险。
方法：收集并分析了100名年龄在26-32岁、有2或3个足月分娩的女性志愿者的静脉血样本。考虑了各种肾上腺素能化合物（激动剂α和β肾上腺素受体，肾上腺素受体阻滞剂）。用乳胶诱导的鲁米诺依赖性化学发光评估中性粒细胞呼吸爆发的强度和肾上腺素能物质对它们的影响。
结果：中性粒细胞活性取决于女性生殖过程的阶段：它在怀孕期间减少，在分娩时达到最低值，并在分娩后的头几个小时显着增加。分娩后的第一天，α-1-肾上腺素能受体在嗜中性粒细胞中高度活跃，NADP-H-氧化酶通过其被活化并形成活化的氧物种。同时,α或β-激动剂抑制细胞的自由基活性。
结论：乳胶诱导的女性血液中性粒细胞的氧化爆发与生殖过程的阶段相关。产后期间的压力状况可以抑制中性粒细胞释放活性氧的能力，这增加了产后感染的风险。
