PDF(Pubmed)
Abstract：
Allelopathy has been considered as a natural method of weed control. Despite the nature of allelochemical compounds has been studied, little is known about the genetic basis underlying allelopathy. However, it is known that rice exhibits diverse allelopathic potentials across varieties, and breeding for rice plants exhibiting allelopathic potential conferring an advantage against weeds in paddy fields would be highly desirable. Knowledge of the gene factors and the identification of the genomic regions responsible for allelopathy would facilitate breeding programs. Taking advantage of the existing genetic diversity in rice, particularly in temperate japonica rice, we conducted a comprehensive investigation into the genetic determinants that contribute to rice allelopathy. Employing Genome-Wide Association Study, we identified four Quantitative Trait Loci, with the most promising loci situated on chromosome 2 and 5. Subsequent inspection of the genes located within these QTLs revealed genes associated with the biosynthesis of secondary metabolites such as Phenylalanine Ammonia Lyase (PAL), a key enzyme in the synthesis of phenolic compounds, and two genes coding for R2R3-type MYB transcription factors. The identification of these two QTLs associated to allelopathy in rice provides a useful tool for further exploration and targeted breeding strategies.
摘要：
化感作用已被认为是控制杂草的自然方法。尽管已经研究了化感化合物的性质，关于化感作用的遗传基础知之甚少。然而,众所周知，水稻在不同品种之间表现出不同的化感潜力，和育种具有化感潜力的水稻植物，赋予稻田中杂草的优势将是非常理想的。了解基因因子和鉴定负责化感作用的基因组区域将有助于育种程序。利用水稻现有的遗传多样性,特别是在温带粳稻中，我们对水稻化感作用的遗传决定因素进行了全面调查。采用全基因组关联研究，我们确定了四个定量特征基因座，最有希望的基因座位于2号和5号染色体上。随后对位于这些QTL内的基因的检查揭示了与次级代谢产物如苯丙氨酸氨裂解酶(PAL)的生物合成相关的基因。酚类化合物合成的关键酶，和两个编码R2R3型MYB转录因子的基因。这两个与水稻化感作用相关的QTL的鉴定为进一步探索和有针对性的育种策略提供了有用的工具。
