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Abstract：
This review highlights the importance of extracellular matrix (ECM) biomaterials in understanding the biology of human trabecular meshwork (TM) and Schlemm\'s canal (SC) cells under normal and simulated glaucoma-like conditions. We provide an overview of recent progress in the development and application of state-of-the-art 3D ECM biomaterials including cell-derived ECM, ECM scaffolds, Matrigel, and ECM hydrogels for studies of TM and SC cell (patho)biology. Such bioengineered platforms enable accurate and reliable modeling of tissue-like cell-cell and cell-ECM interactions. They bridge the gap between conventional 2D approaches and in vivo/ex vivo models, and have the potential to aid in the identification of the causal mechanism(s) for outflow dysfunction in ocular hypertensive glaucoma. We discuss each model\'s benefits and limitations, and close with an outlook on future directions.
摘要：
这篇综述强调了细胞外基质（ECM）生物材料在了解正常和模拟青光眼样条件下人类小梁网（TM）和Schlemm管（SC）细胞生物学方面的重要性。我们概述了包括细胞源性ECM在内的最先进的3DECM生物材料的开发和应用的最新进展，ECM支架,Matrigel,和ECM水凝胶，用于研究TM和SC细胞（病理）生物学。这样的生物工程平台能够准确和可靠地模拟组织样细胞-细胞和细胞-ECM相互作用。它们弥合了传统2D方法和体内/离体模型之间的差距，并有可能帮助确定眼压性青光眼流出功能障碍的病因机制。我们讨论每个模型的优点和局限性，并展望未来方向。
