Abstract：
Flow cytometry remains the most widely used method for detecting and quantifying apoptosis and related forms of cell death in mammalian cells. The multiparametric nature of flow cytometry allows multiple apoptotic characteristics to be labeled and analyzed in a single sample, making it a powerful tool for analyzing the complex progression of apoptotic death. This chapter provides methods for combining assays for single apoptotic characteristics like caspase activation, annexin V binding, and cell membrane permeability into multiparametric assays that provide deeper insights into the cell death process. This approach to analyzing multiple apoptotic characteristics simultaneously yields far more information than single-parameter assays. While more informative than single-parameter assays, these multicolor methods can still be analyzed on relatively simple flow cytometers, making them widely accessible.
摘要：
流式细胞术仍然是用于检测和定量哺乳动物细胞中凋亡和相关形式的细胞死亡的最广泛使用的方法。流式细胞术的多参数性质允许在单个样品中标记和分析多个凋亡特征。使其成为分析凋亡性死亡复杂进展的有力工具。本章提供了结合检测单个细胞凋亡特征如半胱天冬酶激活的方法，膜联蛋白V结合，和细胞膜通透性进入多参数测定，为细胞死亡过程提供更深入的见解。这种分析多个凋亡特征的方法同时产生比单参数测定多得多的信息。虽然信息比单参数测定更多，这些多色方法仍然可以在相对简单的流式细胞仪上进行分析，使它们广泛使用。
