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Abstract：
Uropathogenic Escherichia coli (UPEC) is the most common bacterial agent associated with urinary tract infections, threatening public health systems with elevated medical costs and high morbidity rates. The successful establishment of the infection is associated with virulence factors encoded in its genome, in addition to antibacterial resistance genes, which could limit the treatment and resolution of the infection. In this sense, plant extracts from the genus Echeveria have traditionally been used to treat diverse infectious diseases. However, little is known about the effects of these extracts on bacteria and their potential mechanisms of action. This study aims to sequence a multidrug-resistant UPEC isolate (UTI-U7) and assess the multilocus sequence typing (MLST), virulence factors, antimicrobial resistance profile, genes, serotype, and plasmid content. Antimicrobial susceptibility profiling was performed using the Kirby-Bauer disk diffusion. The antibacterial and anti-adherent effects of the methanol extracts (ME) of Echeveria (E. craigiana, E. kimnachii, and E. subrigida) against UTI-U7 were determined. The isolate was characterized as an O25:H4-B2-ST2279-CH40 subclone and had resistant determinants to aminoglycosides, β-lactams, fluoroquinolones/quinolones, amphenicols, and tetracyclines, which matched with the antimicrobial resistance profile. The virulence genes identified encode adherence factors, iron uptake, protectins/serum resistance, and toxins. Identified plasmids belonged to the IncF group (IncFIA, IncFIB, and IncFII), alongside several prophage-like elements. After an extensive genome analysis that confirmed the pathogenic status of UTI-U7 isolate, Echeveria extracts were tested to determine their antibacterial effects; as an extract, E. subrigida (MIC, 5 mg/mL) displayed the best inhibitory effect. However, the adherence between UTI-U7 and HeLa cells was unaffected by the ME of the E. subrigida extract.
摘要：
尿路致病性大肠杆菌（UPEC）是与尿路感染相关的最常见细菌，以高昂的医疗费用和高发病率威胁公共卫生系统。感染的成功建立与其基因组中编码的毒力因子有关，除了抗菌抗性基因，这可能会限制感染的治疗和解决。在这个意义上,传统上，来自Echeveria属的植物提取物被用于治疗各种传染病。然而,人们对这些提取物对细菌的影响及其潜在的作用机制知之甚少。这项研究旨在测序多药耐药UPEC分离株（UTI-U7）并评估多位点序列分型（MLST），毒力因子，抗菌素耐药性概况，基因，血清型,和质粒含量。使用Kirby-Bauer圆盘扩散进行抗菌素敏感性分析。Echeveria的甲醇提取物（ME）的抗菌和抗粘附作用（E.Craigiana,E.Kimnachii,和E.subrigida）对UTI-U7进行了测定。该分离株的特征为O25：H4-B2-ST2279-CH40亚克隆，并具有对氨基糖苷类的抗性决定簇，β-内酰胺,氟喹诺酮/喹诺酮,氨酚,和四环素,这与抗菌素耐药性概况相匹配。鉴定的毒力基因编码粘附因子，铁吸收，保护素/血清抗性，和毒素。鉴定的质粒属于IncF组(IncFIA，IncFIB,和IncFII),还有几个类似预言的元素。经过广泛的基因组分析，证实UTI-U7分离株的致病状态，对Echeveria提取物进行了测试，以确定其抗菌作用；作为提取物，E.subrigida(MIC，5mg/mL)表现出最好的抑制作用。然而,UTI-U7和HeLa细胞之间的粘附不受E.subrigida提取物的ME影响。
