PDF(Pubmed)
Abstract：
The emu is a novel poultry species in Japan. However, Japanese farmed emu populations have reduced genetic diversity owing to inbreeding. We have previously suggested that there are genetic resources in the Tohoku Safari Park (TSP) and Fuji/Kakegawa Kachoen Garden Park (FGP/KGP) to extend the genetic diversity of commercial emu farms based on microsatellite (SSR) and mitochondrial DNA. However, those markers provide relatively poor information. Thus, we investigated the genetic structure of farmed Japanese populations based on a large-scale genotyping system using RAD-seq and verified the usefulness of TSP and FGP/KGP as genetic resources for expanding genetic diversity. Admixture, phylogenetic, and principal component analyses based on 28,676 SNPs showed that TSP individuals were ancestors in the Okhotsk Emu Farm (OEF). FGP/KGP individuals showed a unique genetic component that differed from that of the others. We have previously reported that the mitochondrial haplotypes of FGP/KGP were shared with an isolated wild population in eastern Australia. These results suggest that FGP/KGP individuals originated from an eastern Australia isolated population different from other populations including ancestral of OEF/TSP. Our results would provide information for the development of Japanese emu farms and industry and for the conservation of genetic resources in the Australian wild emu.
摘要：
the是日本的一种新型家禽。然而,由于近亲繁殖，日本养殖的e种群减少了遗传多样性。我们以前曾建议，东北野生动物园（TSP）和富士/川川Kachoen花园公园（FGP/KGP）中存在遗传资源，以基于微卫星（SSR）和线粒体DNA扩展商业emu农场的遗传多样性。然而,这些标记提供的信息相对较差。因此，我们基于使用RAD-seq的大规模基因分型系统调查了日本养殖种群的遗传结构，并验证了TSP和FGP/KGP作为遗传资源对扩大遗传多样性的有用性。外加剂,系统发育，基于28,676个SNP的主成分分析表明，TSP个体是鄂霍次克Emu农场（OEF）的祖先。FGP/KGP个体表现出与其他个体不同的独特遗传成分。我们以前曾报道过，FGP/KGP的线粒体单倍型与澳大利亚东部的一个孤立的野生种群共享。这些结果表明，FGP/KGP个体起源于澳大利亚东部的孤立种群，与包括OEF/TSP祖先在内的其他种群不同。我们的结果将为日本emu农场和工业的发展以及澳大利亚野生emu的遗传资源保护提供信息。
