Abstract：
BACKGROUND: Hodgsonia heteroclita has been known as an important traditionally consumed medicinal plant of North-East India known to have antidiabetic properties. This study aims to investigate the effects of the ethanolic fruit extract of Hodgsonia heteroclita against hyperglycemia and hyperlipidemia by using streptozotocin (STZ) treated diabetic mice.
METHODS: The fruits of H. heteroclita were collected from the various parts of Kokrajhar district, Assam India (Geographic coordinates: 26°24\'3.85″ N 90°16\'22.30″ E). Basic morphological evaluations were carried out by the Botanical Survey of India, Eastern circle, Shillong, who also certified and identified the plant. Hexane, chloroform, and ethanolic extracts of the fruit of H. heteroclita were investigated for α-amylase inhibition assay as a rapid screening tool for examining anti-diabetic activity. The efficacy of ethanolic extract at a dose of 100, 200, and 300 mg/kg body weight was tested for 21 days in STZ-induced diabetic mice. The body weight, fasting plasma glucose and serum lipids, and hepatic glycogen levels were measured in experimental animals to examine the antihyperglycemic and antihyperlipidemic efficacy of the extract. Both HPTLC and LC-MS analysis was performed to examine the phyotochemicals present in the ethanolic extract of H. heteroclita.
RESULTS: It has been observed that treatment with the ethanolic extract dose-dependently reduced the plasma glucose levels, total cholesterol, low density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol, triglyceride, and increased the body weight, liver glycogens and high-density lipoprotein-cholesterol in STZ treated diabetic mice. HPTLC demonstrated the presence of triterpene compounds and LC-MS analysis revealed the presence Cucurbitacin I, Cucurbitacin E, and Kuguacin G as the triterpene phytoconstituents.
CONCLUSIONS: The present study demonstrated that ethanolic fruit extract of H. heteroclita improved both glycemic and lipid parameters in mice model of diabetes.
METHODS: The fruits of H. heteroclita were collected from the various parts of Kokrajhar district, Assam India (Geographic coordinates: 26°24\'3.85″ N 90°16\'22.30″ E). Basic morphological evaluations were carried out by the Botanical Survey of India, Eastern circle, Shillong, who also certified and identified the plant. Hexane, chloroform, and ethanolic extracts of the fruit of H. heteroclita were investigated for α-amylase inhibition assay as a rapid screening tool for examining anti-diabetic activity. The efficacy of ethanolic extract at a dose of 100, 200, and 300 mg/kg body weight was tested for 21 days in STZ-induced diabetic mice. The body weight, fasting plasma glucose and serum lipids, and hepatic glycogen levels were measured in experimental animals to examine the antihyperglycemic and antihyperlipidemic efficacy of the extract. Both HPTLC and LC-MS analysis was performed to examine the phyotochemicals present in the ethanolic extract of H. heteroclita.
RESULTS: It has been observed that treatment with the ethanolic extract dose-dependently reduced the plasma glucose levels, total cholesterol, low density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol, triglyceride, and increased the body weight, liver glycogens and high-density lipoprotein-cholesterol in STZ treated diabetic mice. HPTLC demonstrated the presence of triterpene compounds and LC-MS analysis revealed the presence Cucurbitacin I, Cucurbitacin E, and Kuguacin G as the triterpene phytoconstituents.
CONCLUSIONS: The present study demonstrated that ethanolic fruit extract of H. heteroclita improved both glycemic and lipid parameters in mice model of diabetes.
摘要：
背景：Hodgsoniaheteroclita被认为是印度东北部一种重要的传统消费药用植物，已知具有抗糖尿病特性。本研究旨在通过使用链脲佐菌素（STZ）治疗糖尿病小鼠，研究霍奇森乙醇果实提取物对高血糖和高脂血症的影响。
方法：从Kokrajhar区的各个地方收集了H.heterclita的果实，阿萨姆印度（地理坐标：26°24\'3.85″N90°16\'22.30″E）。印度植物学调查进行了基本的形态学评估，东方圆圈，西隆,他们还对工厂进行了认证和鉴定。己烷,氯仿,研究了H.heteroplita果实的乙醇提取物，用于α-淀粉酶抑制测定，作为检查抗糖尿病活性的快速筛选工具。在STZ诱导的糖尿病小鼠中测试乙醇提取物在100、200和300mg/kg体重的剂量下的功效21天。体重,空腹血糖和血脂，在实验动物中测量肝糖原水平以检查提取物的抗高血糖和抗高脂血症功效。进行HPTLC和LC-MS分析以检查H.heterchlita的乙醇提取物中存在的植物化学物质。
结果：观察到用乙醇提取物剂量依赖性地降低了血浆葡萄糖水平，总胆固醇,低密度脂蛋白-胆固醇，极低密度脂蛋白胆固醇,甘油三酯，增加了体重，STZ治疗的糖尿病小鼠的肝糖原和高密度脂蛋白胆固醇。HPTLC证明了三萜化合物的存在，LC-MS分析揭示了葫芦素I的存在，葫芦素E,三萜植物成分为库花酸G。
结论：本研究表明，异花果乙醇提取物可改善糖尿病小鼠模型的血糖和血脂参数。
方法：从Kokrajhar区的各个地方收集了H.heterclita的果实，阿萨姆印度（地理坐标：26°24\'3.85″N90°16\'22.30″E）。印度植物学调查进行了基本的形态学评估，东方圆圈，西隆,他们还对工厂进行了认证和鉴定。己烷,氯仿,研究了H.heteroplita果实的乙醇提取物，用于α-淀粉酶抑制测定，作为检查抗糖尿病活性的快速筛选工具。在STZ诱导的糖尿病小鼠中测试乙醇提取物在100、200和300mg/kg体重的剂量下的功效21天。体重,空腹血糖和血脂，在实验动物中测量肝糖原水平以检查提取物的抗高血糖和抗高脂血症功效。进行HPTLC和LC-MS分析以检查H.heterchlita的乙醇提取物中存在的植物化学物质。
结果：观察到用乙醇提取物剂量依赖性地降低了血浆葡萄糖水平，总胆固醇,低密度脂蛋白-胆固醇，极低密度脂蛋白胆固醇,甘油三酯，增加了体重，STZ治疗的糖尿病小鼠的肝糖原和高密度脂蛋白胆固醇。HPTLC证明了三萜化合物的存在，LC-MS分析揭示了葫芦素I的存在，葫芦素E,三萜植物成分为库花酸G。
结论：本研究表明，异花果乙醇提取物可改善糖尿病小鼠模型的血糖和血脂参数。
