Abstract：
Innovative antibacterial therapies using nanomaterials, such as photothermal (PTT) and photodynamic (PDT) treatments, have been developed for treating wound infections. However, creating secure wound dressings with these therapies faces challenges. The primary focus of this study is to prepare an antibacterial nanofiber dressing that effectively incorporates stable loads of functional nanoparticles and demonstrates an efficient synergistic effect between PTT and PDT. Herein, a composite nanofiber mat was fabricated, integrating spherical molybdenum disulfide (MoS2) nanoparticles. MoS2 was deposited onto polylactic acid (PLA) nanofiber mats using vacuum filtration, which was further stabilized by sodium carboxymethyl cellulose (CMC) adhesion and glutaraldehyde (GA) cross-linking. The composite nanofibers demonstrated synergistic antibacterial effects under NIR light irradiation, and the underlying mechanism was explored. They induce bacterial membrane permeability, protein leakage, and intracellular reactive oxygen species (ROS) elevation, ultimately leading to >95 % antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), which is higher than that of single thermotherapy (almost no antibacterial activity) or ROS therapy (about 80 %). In addition, the composite nanofiber mats exhibited promotion effects on infected wound healing in vivo. This study demonstrates the great prospects of composite nanofiber dressings in clinical treatment of bacterial-infected wounds.
摘要：
使用纳米材料的创新抗菌疗法，如光热（PTT）和光动力（PDT）治疗，已被开发用于治疗伤口感染。然而,用这些疗法制造安全的伤口敷料面临挑战。这项研究的主要重点是制备一种抗菌纳米纤维敷料，该敷料有效地掺入了功能纳米颗粒的稳定负载，并证明了PTT和PDT之间的有效协同作用。在这里，制造了复合纳米纤维垫，集成球形二硫化钼（MoS2）纳米颗粒。使用真空过滤将MoS2沉积到聚乳酸（PLA）纳米纤维垫上，通过羧甲基纤维素钠(CMC)粘附和戊二醛(GA)交联进一步稳定。复合纳米纤维在近红外照射下表现出协同抗菌作用，并对其潜在机制进行了探讨。它们诱导细菌膜通透性，蛋白渗漏,和细胞内活性氧（ROS）升高，最终导致对金黄色葡萄球菌的抗菌活性>95%(S.金黄色葡萄球菌)和大肠杆菌(E.大肠杆菌)，高于单一热疗（几乎没有抗菌活性）或ROS治疗（约80％）。此外,复合纳米纤维垫对体内感染伤口愈合具有促进作用。本研究展示了复合纳米纤维敷料在临床治疗细菌感染创面中的巨大前景。
