Abstract：
Bacteria and fungi are ubiquitous throughout built environments and are suspended in the air, potentially affecting human health. However, the impacts of climate zones on the diversity, structure, and stochastic assembly of indoor airborne microbes remain unknown. This study comprehensively analyzed indoor airborne microbes across five climate zones in China during the summer and winter using high-throughput sequencing. The diversity and structure of indoor airborne communities vary across climatic zones. A random forest model was used to identify biomarkers in different climate zones. The results showed no relationship between the biomarkers and their rankings in mean relative abundance. The Sloan neutral model fitting results indicated that the impact of climate zones on the stochastic process in the assembly of indoor airborne microbes was considerably more important than that of seasons. Additionally, the influence of seasons on the diversity, structure, and stochastic assembly process of indoor airborne microbes differed among different climate zones. The diversity, structure, and stochastic assembly processes of bacteria present distinctive outcomes in climate zones and seasons compared with those of fungi. Overall, these findings indicate that customized strategies are necessary to manage indoor airborne microbial communities in each climate zone, season, and for specific microbial species.
摘要：
细菌和真菌在整个建筑环境中无处不在，并悬浮在空气中，有可能影响人类健康。然而,气候区对多样性的影响，结构,室内空气微生物的随机组装仍然未知。本研究利用高通量测序技术全面分析了中国5个气候带夏季和冬季的室内空气传播微生物。室内空气传播群落的多样性和结构因气候带而异。使用随机森林模型来识别不同气候区的生物标志物。结果显示生物标志物与其平均相对丰度的排名之间没有关系。斯隆中性模型的拟合结果表明，气候区对室内空气微生物组装随机过程的影响比季节对随机过程的影响要重要得多。此外,季节对多样性的影响，结构,室内空气微生物的随机组装过程在不同气候区之间存在差异。多样性，结构,与真菌相比，细菌的随机组装过程在气候区和季节表现出独特的结果。总的来说,这些发现表明，定制策略对于管理每个气候区的室内空气微生物群落是必要的，季节,和特定的微生物物种。
