PDF(Pubmed)
Abstract：
BACKGROUND: Recent studies point to the need to incorporate the detection of non-falciparum species into malaria surveillance activities in sub-Saharan Africa, where 95% of the world\'s malaria cases occur. Although malaria caused by infection with Plasmodium falciparum is typically more severe than malaria caused by the non-falciparum Plasmodium species P. malariae, P. ovale spp. and P. vivax, the latter may be more challenging to diagnose, treat, control and ultimately eliminate. The prevalence of non-falciparum species throughout sub-Saharan Africa is poorly defined. Tanzania has geographical heterogeneity in transmission levels but an overall high malaria burden.
METHODS: To estimate the prevalence of malaria species in Mainland Tanzania, we randomly selected 1428 samples from 6005 asymptomatic isolates collected in previous cross-sectional community surveys across four regions and analyzed these by quantitative PCR to detect and identify the Plasmodium species.
RESULTS: Plasmodium falciparum was the most prevalent species in all samples, with P. malariae and P. ovale spp. detected at a lower prevalence (< 5%) in all four regions; P. vivax was not detected in any sample.
CONCLUSIONS: The results of this study indicate that malaria elimination efforts in Tanzania will need to account for and enhance surveillance of these non-falciparum species.
METHODS: To estimate the prevalence of malaria species in Mainland Tanzania, we randomly selected 1428 samples from 6005 asymptomatic isolates collected in previous cross-sectional community surveys across four regions and analyzed these by quantitative PCR to detect and identify the Plasmodium species.
RESULTS: Plasmodium falciparum was the most prevalent species in all samples, with P. malariae and P. ovale spp. detected at a lower prevalence (< 5%) in all four regions; P. vivax was not detected in any sample.
CONCLUSIONS: The results of this study indicate that malaria elimination efforts in Tanzania will need to account for and enhance surveillance of these non-falciparum species.
摘要：
背景：最近的研究指出，需要将非恶性疟原虫物种的检测纳入撒哈拉以南非洲的疟疾监测活动，世界上95%的疟疾病例发生在那里。尽管由恶性疟原虫感染引起的疟疾通常比由非恶性疟原虫引起的疟疾更为严重。P.Ovalespp.和间日疟原虫,后者可能对诊断更具挑战性，请客,控制并最终消除。整个撒哈拉以南非洲地区的非恶性疟原虫物种的流行程度尚不明确。坦桑尼亚的传播水平具有地理异质性，但总体上疟疾负担很高。
方法：为了估计坦桑尼亚大陆的疟疾流行情况，我们从之前在4个地区进行的横断面社区调查中收集的6005个无症状分离株中随机选取了1428个样本,并通过定量PCR对这些样本进行了分析,以检测和鉴定疟原虫种类.
结果：恶性疟原虫是所有样本中最普遍的物种，与疟原虫和卵卵圆虫属。在所有四个地区均以较低的患病率（<5％）检测到；在任何样本中均未检测到间日疟原虫。
结论：这项研究的结果表明，坦桑尼亚消除疟疾的努力将需要考虑并加强对这些非恶性疟原虫物种的监测。
方法：为了估计坦桑尼亚大陆的疟疾流行情况，我们从之前在4个地区进行的横断面社区调查中收集的6005个无症状分离株中随机选取了1428个样本,并通过定量PCR对这些样本进行了分析,以检测和鉴定疟原虫种类.
结果：恶性疟原虫是所有样本中最普遍的物种，与疟原虫和卵卵圆虫属。在所有四个地区均以较低的患病率（<5％）检测到；在任何样本中均未检测到间日疟原虫。
结论：这项研究的结果表明，坦桑尼亚消除疟疾的努力将需要考虑并加强对这些非恶性疟原虫物种的监测。
