Abstract：
The Sceletium-type alkaloids, known for their anxiolytic and antidepressant activities, have been recently found to be biosynthesized in Narcissus cv. Hawera, which is largely used as an ornamental plant. An alkaloid fraction enriched with Sceletium-type alkaloids from the plant has shown promising antidepressant and anxiolytic activities. In the present study, qualitative and quantitative analyses of the alkaloids in the plant organs were performed during one vegetation season by GC-MS. The alkaloid pattern and total alkaloid content was found to depend strongly on the stage of development and plant organ. The alkaloid content of bulbs was found to be highest during the dormancy period and lowest in sprouting bulbs. The leaves showed the highest alkaloid content during the intensive vegetative growth and lowest during flowering. In total, 13 alkaloids were detected in the methanol extracts of Narcissus cv. Hawera, six Sceletium-type and seven typical Amaryllidaceae alkaloids. Major alkaloids in the alkaloid pattern were lycorine, 6-epi-mesembrenol, mesembrenone, sanguinine, and galanthamine. The leaves of flowering plants were found to have the highest amount of 6-epi-mesembrenol. Mesembrenone was found to be dominant alkaloid in the leaves of sprouting bulbs and in the flowers. Considering the biomass of the plant, the dormant bulbs are the best source of alkaloid fractions enriched with 6-epi-mesembrenol. The flowers and the young leaves can be used for preparation of alkaloid fractions enriched with mesembrenone. The results indicates that Narcissus cv. Hawera is an emerging source of valuable bioactive compounds and its utilization can be extended as a medicinal plant.
摘要：
Sceletium型生物碱，以其抗焦虑和抗抑郁活性而闻名，最近发现水仙cv是生物合成的。Hawera,主要用作观赏植物。来自植物的富含Sceletium型生物碱的生物碱部分显示出有希望的抗抑郁和抗焦虑活性。在本研究中,通过GC-MS在一个植被季节对植物器官中的生物碱进行了定性和定量分析。发现生物碱模式和总生物碱含量在很大程度上取决于发育阶段和植物器官。球茎的生物碱含量在休眠期最高，而在发芽球茎中最低。在密集的营养生长期间，叶片显示出最高的生物碱含量，而在开花期间则最低。总的来说,在水仙的甲醇提取物中检测到13种生物碱。Hawera,6种Sceletium型和7种典型的石蒜科生物碱。生物碱模式中的主要生物碱是石蒜碱，6-epi-mesembrenol,Mesembrenone,血统，和加兰他敏.发现开花植物的叶子中6-epi-mesembrenol的含量最高。在发芽的鳞茎的叶子和花朵中，发现了美森烯酮是主要的生物碱。考虑到植物的生物量，休眠的鳞茎是富含6-epi-mesembrenol的生物碱级分的最佳来源。花和幼叶可用于制备富含美沙烯酮的生物碱部分。结果表明，水仙cv。Hawera是有价值的生物活性化合物的新兴来源，其用途可以扩展为药用植物。
