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Abstract：
Nanotechnology for tumor diagnosis and optical therapy has attracted widespread interest due to its low toxicity and convenience but is severely limited due to uncontrollable tumor targeting. In this work, homologous cancer cell membrane-camouflaged multifunctional hybrid metal coordination nanoparticles (DRu/Gd@CM) were prepared for MRI-guided photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors. Bimetallic coordination nanoparticles are composed of three functional modules: dopamine, Ru(dcbpy)3Cl2 and GdCl3, which are connected through 1,4-Bis[(1H-imidazole-1-yl)methyl]benzene (BIX). Their morphology can be easily controlled by adjusting the ratio of precursors. Optimistically, the intrinsic properties of the precursors, including the photothermal properties of polydopamine (PDA), the magnetic resonance (MR) response of Gd3+, and the singlet oxygen generation of Ru(dcbpy)3Cl2, are well preserved in the hybrid metal nanoparticles. Furthermore, the targeting of homologous cancer cell membranes enables these coordinated nanoparticles to precisely target tumor cells. The MR imaging capabilities and the combination of PDT and PTT were demonstrated in in vitro experiments. In addition, in vivo experiments indicated that the nanoplatform showed excellent tumor accumulation and therapeutic effects on mice with subcutaneous tumors, and could effectively eliminate tumors within 14 days. Therefore, it expanded the new horizon for the preparation of modular nanoplatform and imaging-guided optical therapy of tumors.
摘要：
用于肿瘤诊断和光学治疗的纳米技术由于其低毒性和便利性而引起了广泛的兴趣，但由于无法控制的肿瘤靶向而受到严重限制。在这项工作中,制备了同源癌细胞膜伪装的多功能混合金属配位纳米粒子（DRu/Gd@CM），用于MRI引导的肿瘤光动力治疗（PDT）和光热治疗（PTT）。双金属配位纳米粒子由三个功能模块组成：多巴胺，Ru(dcbpy)3Cl2和GdCl3,它们通过1,4-双[(1H-咪唑-1-基)甲基]苯(BIX)连接。通过调节前体的比例可以容易地控制它们的形态。乐观地,前体的内在性质，包括聚多巴胺（PDA）的光热特性，Gd3+的磁共振（MR）响应，和Ru(dcbpy)3Cl2的单线态氧生成在杂化金属纳米粒子中得到很好的保存。此外，同源癌细胞膜的靶向使这些协调的纳米颗粒能够精确靶向肿瘤细胞。在体外实验中证明了MR成像能力以及PDT和PTT的组合。此外,体内实验表明，纳米平台对皮下肿瘤小鼠表现出优异的肿瘤积累和治疗效果，并能在14天内有效消除肿瘤。因此,它为制备模块化纳米平台和成像引导的肿瘤光学治疗拓展了新的视野。
