Abstract：
Research indicates that long noncoding RNAs (lncRNAs) contribute significantly to fibrotic diseases. Although lncRNAs may play a role in hypertrophic scars after burns, its mechanisms remain poorly understood.
Using chip technology, we compared the lncRNA expression profiles of burn patients and healthy controls (HCs). Microarray results were examined by quantitative reverse-transcription polymerase chain reaction (RT-PCR) to verify their reliability. The biological functions of differentially expressed mRNAs and the relationships between genes and signaling pathways were investigated by Gene Ontology (GO) and pathway analyses, respectively.
In contrast with HCs, it was found that 2738 lncRNAs (1628 upregulated) and 2166 mRNAs (1395 upregulated) were differentially expressed in hypertrophic scars after burn. Results from RT-PCR were consistent with those from microarray. GO and pathway analyses revealed that the differentially expressed mRNAs are mainly associated with processes related to cytokine secretion in the immune system, notch signaling, and MAPK signaling.
The lncRNA expression profiles of hypertrophic scars after burn changed significantly compared with HCs. It was believed that the transcripts could be used as potential targets for inhibiting abnormal scar formation in burn patients.
Using chip technology, we compared the lncRNA expression profiles of burn patients and healthy controls (HCs). Microarray results were examined by quantitative reverse-transcription polymerase chain reaction (RT-PCR) to verify their reliability. The biological functions of differentially expressed mRNAs and the relationships between genes and signaling pathways were investigated by Gene Ontology (GO) and pathway analyses, respectively.
In contrast with HCs, it was found that 2738 lncRNAs (1628 upregulated) and 2166 mRNAs (1395 upregulated) were differentially expressed in hypertrophic scars after burn. Results from RT-PCR were consistent with those from microarray. GO and pathway analyses revealed that the differentially expressed mRNAs are mainly associated with processes related to cytokine secretion in the immune system, notch signaling, and MAPK signaling.
The lncRNA expression profiles of hypertrophic scars after burn changed significantly compared with HCs. It was believed that the transcripts could be used as potential targets for inhibiting abnormal scar formation in burn patients.
摘要：
目的：研究表明长链非编码RNA（lncRNAs）对纤维化疾病有重要贡献。尽管lncRNAs可能在烧伤后的肥厚性瘢痕中发挥作用，其机制仍然知之甚少。
方法：使用芯片技术，我们比较了烧伤患者和健康对照(HCs)的lncRNA表达谱.通过定量逆转录聚合酶链反应（RT-PCR）检查微阵列结果以验证其可靠性。通过基因本体论(GO)和通路分析研究差异表达mRNA的生物学功能以及基因与信号通路之间的关系,分别。
结果：与HC相比，发现2738个lncRNAs（1628个上调）和2166个mRNAs（1395个上调）在烧伤后的增生性瘢痕中差异表达。RT-PCR的结果与微阵列的结果一致。GO和通路分析显示,差异表达的mRNA主要与免疫系统中细胞因子分泌相关,陷波信号,和MAPK信号。
结论：与HCs相比，烧伤后肥厚性瘢痕的lncRNA表达谱发生了显著变化。认为转录本可以用作抑制烧伤患者异常瘢痕形成的潜在靶标。
方法：使用芯片技术，我们比较了烧伤患者和健康对照(HCs)的lncRNA表达谱.通过定量逆转录聚合酶链反应（RT-PCR）检查微阵列结果以验证其可靠性。通过基因本体论(GO)和通路分析研究差异表达mRNA的生物学功能以及基因与信号通路之间的关系,分别。
结果：与HC相比，发现2738个lncRNAs（1628个上调）和2166个mRNAs（1395个上调）在烧伤后的增生性瘢痕中差异表达。RT-PCR的结果与微阵列的结果一致。GO和通路分析显示,差异表达的mRNA主要与免疫系统中细胞因子分泌相关,陷波信号,和MAPK信号。
结论：与HCs相比，烧伤后肥厚性瘢痕的lncRNA表达谱发生了显著变化。认为转录本可以用作抑制烧伤患者异常瘢痕形成的潜在靶标。
