PDF(Pubmed)
Abstract：
Fungal secondary metabolites are not necessary for growth, but they are important for fungal metabolism and ecology because they provide selective advantages for competition, survival and interactions with the environment. These various metabolites are widely used as medicinal precursors and insecticides. Secondary metabolism genes are commonly arranged in clusters along chromosomes, which allow for the coordinate control of complete pathways. In this study, we created the Fungal Gene Cluster Database to store, retrieve, and visualize secondary metabolite gene cluster information across fungal species. The database was created by merging data from RNA sequencing, Basic Local Alignment Search Tool, genome browser, enrichment analysis and the R Shiny web framework to visualize and query putative gene clusters. This database facilitated the rapid and thorough examination of significant gene clusters across fungal species by detecting, defining and graphically displaying the architecture, organization and expression patterns of secondary metabolite gene clusters. In general, this genomic resource makes use of the tremendous chemical variety of the products of these ecologically and biotechnologically significant gene clusters to our further understanding of fungal secondary metabolism. Database URL: https://www.hebaubioinformatics.cn/FungalGeneCluster/.
摘要：
真菌次生代谢产物不是生长所必需的,但它们对真菌代谢和生态学很重要，因为它们为竞争提供了选择性优势，生存和与环境的互动。这些各种代谢物被广泛用作药物前体和杀虫剂。次级代谢基因通常沿着染色体成簇排列，允许协调控制完整的路径。在这项研究中,我们创建了真菌基因簇数据库来存储,检索,并可视化真菌物种的次生代谢物基因簇信息。数据库是通过合并来自RNA测序的数据创建的，基本局部路线搜索工具，基因组浏览器，富集分析和RShiny网络框架来可视化和查询推定的基因簇。该数据库通过检测，促进了跨真菌物种的重要基因簇的快速和彻底的检查，定义并以图形方式显示体系结构，次级代谢产物基因簇的组织和表达模式。总的来说,这种基因组资源利用这些具有生态和生物技术意义的基因簇产物的巨大化学多样性来进一步了解真菌的次级代谢。数据库URL：https://www。hebaubioinformatics.cn/FungalGeneCluster/。
