Abstract：
The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary immune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can be differentiated into immune-like cells for use in reproductive biology experiments. In this chapter, we discussed cell culture methods of maintaining and differentiating HL-60, THP-1, and NK-92 cells to obtain neutrophil-like, macrophage-like, and decidual natural killer-like cells, respectively, which can then be used together with intrauterine cells to elucidate and investigate immune mechanisms that contribute to parturition.
摘要：
导致人类分娩的炎症过程主要是由免疫细胞促进的，可以通过从胎儿-母体界面分离和表征原代免疫细胞来研究。然而,免疫细胞采样方法的困难和不一致以及体外寿命短阻止了它们在机理研究中的使用，以了解母胎免疫生物学。为了解决这些限制，现有的细胞系模型可以分化为免疫样细胞，用于生殖生物学实验。在这一章中,我们讨论了维持和分化HL-60，THP-1和NK-92细胞以获得中性粒细胞样的细胞培养方法，巨噬细胞样，和蜕膜类自然杀伤细胞，分别,然后可以与子宫内细胞一起使用，以阐明和研究有助于分娩的免疫机制。
