Abstract：
During human pregnancy, leukocytes that infiltrate the maternal-fetal interface play a major role in establishing a delicate balance between immune tolerance and functional response and setting the inflammatory process that leads to labor. Here we describe two methods for isolating immune cells from the chorioamniotic membranes (decidua parietalis) and placental blood (decidua basalis) that combine gentle enzymatic digestion, magnetic cell sorting, and density gradient. Isolated leukocytes can be immunophenotypified by flow cytometry, and both isolation methods are compatible with downstream cellular and molecular applications, such as cell culture, transcriptome, and proteome analyses.
摘要：
在人类怀孕期间,渗入母胎界面的白细胞在建立免疫耐受和功能反应之间的微妙平衡以及设定导致分娩的炎症过程中起着重要作用。在这里，我们描述了从绒毛膜羊膜（小蜕膜）和胎盘血液（基底蜕膜）中分离免疫细胞的两种方法，这些方法结合了温和的酶消化，磁性细胞分选，和密度梯度。分离的白细胞可以通过流式细胞术进行免疫分型，两种分离方法都与下游细胞和分子应用兼容，如细胞培养，转录组，和蛋白质组分析。
