PDF(Pubmed)
Abstract：
UNASSIGNED: The nonenzymatic glycation of fibroblasts causes functional downregulation and behavioral disorders in the skin.
UNASSIGNED: To investigate the effect of Inonotus obliquus on the nonenzymatic glycation of skin, we examined the inhibition of advanced glycation end products (AGEs) using four extraction methods: n-butanol, ethyl acetate, n-hexane and aqueous alcohol precipitation. The physical properties and chemical structure of the most effective, purified, crude I. obliquus polysaccharide (IOP) were examined. The effects of IOP on carboxymethyl lysine (CML) accumulation, inflammatory factor release, reactive oxygen species (ROS) production, key extracellular matrix (ECM) protein (MMP 1, 2 and 9; FN-1, LM-5 and COL-1) mRNA expression, and cell survival, migration and adhesion were also examined via cellular assays.
UNASSIGNED: IOP is a polysaccharide with a molecular weight (Mw) of 2.396 × 104 (±6.626%) that is composed mainly of glucose, galactose, xylose, mannose and arabinose (29.094:21.705:14.857:9.375:7.709). In addition, a cellular antiglycation assay showed that IOP, which can promote ECM formation by inhibiting the accumulation of CML, inhibiting the release of inflammatory factors (IL-1β, IL-6, and TNF-α), inhibiting the production of reactive oxygen species (ROS), inhibiting the expression of matrix metalloproteinases (MMP-1\\-2\\-9), promoting the synthesis of ECMs (COL1, FN1, and LM5), and improving cellular dysfunction, had strong antiglycation activity at concentrations in the range of 6-24 μg/mL.
UNASSIGNED: IOP effectively reduced the levels of inflammatory factors and reactive oxygen species produced by AGEs, further preventing the impairment of cell behavior (decreased migration and reduced cell adhesion) and preventing the downregulation of the expression of key extracellular matrix proteins induced by AGEs. The results indicate the potential application of IOP as an AGE inhibitor in skin care.
UNASSIGNED: To investigate the effect of Inonotus obliquus on the nonenzymatic glycation of skin, we examined the inhibition of advanced glycation end products (AGEs) using four extraction methods: n-butanol, ethyl acetate, n-hexane and aqueous alcohol precipitation. The physical properties and chemical structure of the most effective, purified, crude I. obliquus polysaccharide (IOP) were examined. The effects of IOP on carboxymethyl lysine (CML) accumulation, inflammatory factor release, reactive oxygen species (ROS) production, key extracellular matrix (ECM) protein (MMP 1, 2 and 9; FN-1, LM-5 and COL-1) mRNA expression, and cell survival, migration and adhesion were also examined via cellular assays.
UNASSIGNED: IOP is a polysaccharide with a molecular weight (Mw) of 2.396 × 104 (±6.626%) that is composed mainly of glucose, galactose, xylose, mannose and arabinose (29.094:21.705:14.857:9.375:7.709). In addition, a cellular antiglycation assay showed that IOP, which can promote ECM formation by inhibiting the accumulation of CML, inhibiting the release of inflammatory factors (IL-1β, IL-6, and TNF-α), inhibiting the production of reactive oxygen species (ROS), inhibiting the expression of matrix metalloproteinases (MMP-1\\-2\\-9), promoting the synthesis of ECMs (COL1, FN1, and LM5), and improving cellular dysfunction, had strong antiglycation activity at concentrations in the range of 6-24 μg/mL.
UNASSIGNED: IOP effectively reduced the levels of inflammatory factors and reactive oxygen species produced by AGEs, further preventing the impairment of cell behavior (decreased migration and reduced cell adhesion) and preventing the downregulation of the expression of key extracellular matrix proteins induced by AGEs. The results indicate the potential application of IOP as an AGE inhibitor in skin care.
摘要：
■成纤维细胞的非酶糖基化导致皮肤中的功能下调和行为障碍。
■为了研究褐孔菌对皮肤非酶糖基化的影响，我们使用四种提取方法检查了晚期糖基化终产物（AGEs）的抑制作用：正丁醇，乙酸乙酯,正己烷和含水醇沉淀。最有效的物理性质和化学结构，纯化,检测了粗I.斜齿多糖(IOP)。眼压对羧甲基赖氨酸（CML）积累的影响，炎症因子释放，活性氧（ROS）的产生，关键的细胞外基质（ECM）蛋白（MMP1，2和9；FN-1，LM-5和COL-1）mRNA表达，和细胞存活，还通过细胞测定检查迁移和粘附。
■IOP是一种分子量（Mw）为2.396×104（±6.626％）的多糖，主要由葡萄糖组成，半乳糖,木糖,甘露糖和阿拉伯糖（29.094：21.705：14.857：9.375：7.709）。此外,细胞抗糖基化试验显示，可以通过抑制CML的积累来促进ECM的形成，抑制炎症因子的释放(IL-1β，IL-6和TNF-α），抑制活性氧（ROS）的产生，抑制基质金属蛋白酶（MMP-1\\-2\\-9）的表达，促进ECMs（COL1，FN1和LM5）的合成，改善细胞功能障碍，在6-24μg/mL的浓度范围内具有强的抗糖基化活性。
■IOP有效降低了AGEs产生的炎症因子和活性氧的水平，进一步防止细胞行为的损害（迁移减少和细胞粘附减少），并防止AGEs诱导的关键细胞外基质蛋白表达下调。结果表明IOP作为AGE抑制剂在皮肤护理中的潜在应用。
■为了研究褐孔菌对皮肤非酶糖基化的影响，我们使用四种提取方法检查了晚期糖基化终产物（AGEs）的抑制作用：正丁醇，乙酸乙酯,正己烷和含水醇沉淀。最有效的物理性质和化学结构，纯化,检测了粗I.斜齿多糖(IOP)。眼压对羧甲基赖氨酸（CML）积累的影响，炎症因子释放，活性氧（ROS）的产生，关键的细胞外基质（ECM）蛋白（MMP1，2和9；FN-1，LM-5和COL-1）mRNA表达，和细胞存活，还通过细胞测定检查迁移和粘附。
■IOP是一种分子量（Mw）为2.396×104（±6.626％）的多糖，主要由葡萄糖组成，半乳糖,木糖,甘露糖和阿拉伯糖（29.094：21.705：14.857：9.375：7.709）。此外,细胞抗糖基化试验显示，可以通过抑制CML的积累来促进ECM的形成，抑制炎症因子的释放(IL-1β，IL-6和TNF-α），抑制活性氧（ROS）的产生，抑制基质金属蛋白酶（MMP-1\\-2\\-9）的表达，促进ECMs（COL1，FN1和LM5）的合成，改善细胞功能障碍，在6-24μg/mL的浓度范围内具有强的抗糖基化活性。
■IOP有效降低了AGEs产生的炎症因子和活性氧的水平，进一步防止细胞行为的损害（迁移减少和细胞粘附减少），并防止AGEs诱导的关键细胞外基质蛋白表达下调。结果表明IOP作为AGE抑制剂在皮肤护理中的潜在应用。
