Abstract：
Aquatic fishes are threatened by the strong pathogenic bacterium Nocardia seriolae, which challenges the current prevention and treatment approaches. This study introduces luminogens with aggregation-induced emission (AIE) as an innovative and non-antibiotic therapy for N. seriolae. Specifically, the AIE photosensitizer, TTCPy-3 is employed against N. seriolae. We evaluated the antibacterial activity of TTCPy-3 and investigated the killing mechanism against N. seriolae, emphasizing its ability to aggregate within the bacterium and produce reactive oxygen species (ROS). TTCPy-3 could effectively aggregate in N. seriolae, generate ROS, and perform real-time imaging of the bacteria. A bactericidal efficiency of 100% was observed while concentrations exceeding 4 μM in the presence of white light irradiation for 10 min. In vivo, evaluation on zebrafish (Danio rerio) confirmed the superior therapeutic efficacy induced by TTCPy-3 to fight against N. seriolae infections. TTCPy-3 offers a promising strategy for treating nocardiosis of fish, paving the way for alternative treatments beyond traditional antibiotics and potentially addressing antibiotic resistance.
摘要：
水生鱼类受到强烈致病菌诺卡氏菌的威胁，这对当前的预防和治疗方法提出了挑战。这项研究引入了具有聚集诱导发射（AIE）的发光体，作为一种创新的非抗生素疗法。具体来说,AIE光敏剂，TTCPy-3用于对抗N.seriolae。我们评估了TTCPy-3的抗菌活性，并研究了其对N.seriolae的杀伤机制。强调其在细菌内聚集并产生活性氧（ROS）的能力。TTCPy-3可以有效地聚集在N.seriolae中，生成ROS，并对细菌进行实时成像。在白光照射10分钟的情况下，当浓度超过4μM时，观察到杀菌效率为100％。在体内,对斑马鱼（Daniorerio）的评估证实了TTCPy-3对对抗N.seriolae感染的优异治疗效果。TTCPy-3提供了一种有前途的策略，用于治疗鱼类的诺卡尼病，为传统抗生素之外的替代疗法铺平道路，并有可能解决抗生素耐药性问题。
