Abstract：
The aim of this study was the identification of luteolin in Prosopis farcta extract (PFE) and melatonin to evaluate its effect on THC withdrawal syndrome in mice. Luteolin was identified by high-performance liquid chromatography (HPCL). Signs of toxicity of mice in PFE and luteolin were monitored for LD50 calculation. The behavioral symptoms of THC withdrawal (stereotypies, ambulation, and inactivity time) induced by the rimonabant challenge were illustrated in THC-dependent mice receiving PFE, luteolin, and melatonin. The expression of mature BDNF (mBDNF) was evaluated by Western blot analysis. The dopamine concentrations were measured using HPLC. PFE and luteolin LD50 were 650 and 220 mg/kg, respectively. PFE (300 mg/kg), all doses of luteolin, and melatonin increased significantly the mBDNF expression and decreased the dopamine concentration. The findings suggest that PFE, luteolin, and melatonin are mighty in reducing the signs of THC withdrawal. It seems these effects were due to a decrease in dopamine concentration level and an increase in mBDNF protein expression in mice brains.
摘要：
本研究的目的是鉴定Prosopisfarcta提取物（PFE）和褪黑激素中的木犀草素，以评估其对小鼠THC戒断综合征的影响。木犀草素通过高效液相色谱法(HPCL)鉴定。监测小鼠在PFE和木犀草素中的毒性迹象以进行LD50计算。THC戒断的行为症状(刻板印象，步行,和不活动时间）在接受PFE的THC依赖性小鼠中说明了利莫那班攻击引起的，木犀草素,还有褪黑激素.通过蛋白质印迹分析评估成熟BDNF(mBDNF)的表达。使用HPLC测量多巴胺浓度。PFE和木犀草素的LD50分别为650和220mg/kg，分别。PFE（300mg/kg），所有剂量的木犀草素，褪黑素显著增加mBDNF表达，降低多巴胺浓度。研究结果表明，PFE，木犀草素,褪黑激素在减少四氢大麻酚戒断的迹象方面非常强大。这些效应似乎是由于小鼠脑中多巴胺浓度水平的降低和mBDNF蛋白表达的增加。
