Abstract：
Fluoride (F) has been employed worldwide to control dental caries. More recently, it has been suggested that the consumption of low doses of F in the drinking water may reduce blood glucose levels, introducing a new perspective for the use of F for the management of blood glucose. However, the exact mechanism by which F affects blood glucose levels remains largely unexplored. Given that the small gut plays a pivotal role in glucose homeostasis, the aim of this study was to investigate the proteomic changes induced by low doses of F in the ileum of female nonobese-diabetic (NOD) mice. Forty-two female NOD mice were divided into two groups based on the F concentration in their drinking water for 14 weeks: 0 (control) or 10 mgF/L. At the end of the experimental period, the ileum was collected for proteomic and Western blot analyses. Proteomic analysis indicated an increase in isoforms of actin, gastrotropin, several H2B histones, and enzymes involved in antioxidant processes, as well as a decrease in enzymes essential for energy metabolism. In summary, our data indicates an adaptive response of organism to preserve protein synthesis in the ileum, despite significant alterations in energy metabolism typically induced by F, therefore highlighting the safety of controlled fluoridation in water supplies.
摘要：
氟化物(F)已在世界范围内用于控制龋齿。最近,有人认为，在饮用水中消耗低剂量的F可以降低血糖水平，为使用F管理血糖提供了新的视角。然而,F影响血糖水平的确切机制在很大程度上仍未被探索。鉴于小肠在葡萄糖稳态中起着关键作用，这项研究的目的是研究低剂量F在雌性非肥胖糖尿病（NOD）小鼠回肠中引起的蛋白质组学变化。根据饮用水中的F浓度将42只雌性NOD小鼠分为两组，持续14周：0（对照）或10mgF/L。在实验期结束时,收集回肠进行蛋白质组学和Western印迹分析.蛋白质组分析表明肌动蛋白的同工型增加，胃泌素,几个H2B组蛋白，和参与抗氧化过程的酶，以及能量代谢所必需的酶的减少。总之,我们的数据表明生物体对保持回肠蛋白质合成的适应性反应，尽管通常由F引起的能量代谢发生了显着变化，因此强调了供水中受控氟化的安全性。
