PDF(Pubmed)
Abstract：
UNASSIGNED: Dengue is the most common arboviral infection that spreads by Aedes aegypti and Aedes albopictus mosquitoes, and is quickly gaining prominence as a major mosquito-borne viral disease. One of the major public health issues is dengue fever (DF), which can also cause dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Therefore, this study focused on comparison of dengue antigen non-structural protein (NS1) and immunoglobulin M (IgM) using enzyme-linked immunosorbent assay (ELISA) and immunochromatography test (ICT) for detection of dengue.
UNASSIGNED: In a Tertiary Care Hospital (TCH), sociodemographic status of probable dengue cases from February 2021 to February 2022 was studied. The results of the Dengue Antigen NS1 and IgM ICT, Dengue NS1 Microlisa, and Dengue IgM Microlisa were compared in order to determine the effective one at managing patients and preventing complications like DHF and DSS.
UNASSIGNED: In distribution of 100 ICT reactive samples, 50% were NS1 reactive and 50% were IgM reactive. One hundred ICT reactive samples were further processed for IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for both NS1 and IgM. Sensitivity and specificity of NS1 ICT were 89.3% and 71.4% and that of IgM ICT was 88% and 64.5%. As a result of antigenic cross-reactivity, false positive cases were reported. Platelet count of the patients was correlated with an optical density (OD) value of ELISA for both NS1 and IgM. In the present study, patients having low platelet count showed high OD value.
UNASSIGNED: In cases of severe thrombocytopenia (platelet count <50000), early diagnosis by screening ICT and confirmation by ELISA (NS1 and IgM) would reduce the complications like DHF and DSS.
UNASSIGNED: In a Tertiary Care Hospital (TCH), sociodemographic status of probable dengue cases from February 2021 to February 2022 was studied. The results of the Dengue Antigen NS1 and IgM ICT, Dengue NS1 Microlisa, and Dengue IgM Microlisa were compared in order to determine the effective one at managing patients and preventing complications like DHF and DSS.
UNASSIGNED: In distribution of 100 ICT reactive samples, 50% were NS1 reactive and 50% were IgM reactive. One hundred ICT reactive samples were further processed for IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for both NS1 and IgM. Sensitivity and specificity of NS1 ICT were 89.3% and 71.4% and that of IgM ICT was 88% and 64.5%. As a result of antigenic cross-reactivity, false positive cases were reported. Platelet count of the patients was correlated with an optical density (OD) value of ELISA for both NS1 and IgM. In the present study, patients having low platelet count showed high OD value.
UNASSIGNED: In cases of severe thrombocytopenia (platelet count <50000), early diagnosis by screening ICT and confirmation by ELISA (NS1 and IgM) would reduce the complications like DHF and DSS.
摘要：
登革热是由埃及伊蚊和白纹伊蚊传播的最常见的虫媒病毒感染，并迅速成为一种主要的蚊媒病毒性疾病。主要的公共卫生问题之一是登革热（DF），这也可能导致登革热出血热（DHF）和登革热休克综合征（DSS）。因此,这项研究的重点是使用酶联免疫吸附试验（ELISA）和免疫层析试验（ICT）检测登革热抗原非结构蛋白（NS1）和免疫球蛋白M（IgM）进行比较。
■在三级护理医院（TCH），研究了2021年2月至2022年2月可能登革热病例的社会人口统计学状况.登革热抗原NS1和IgMICT的结果，登革热NS1Microlisa,比较了登革热IgMMicrolisa，以确定有效管理患者和预防DHF和DSS等并发症的方法。
■在100个ICT反应样品的分布中，50%为NS1反应性,50%为IgM反应性。进一步处理了100个ICT反应样品，用于NS1和IgM的IgM抗体捕获酶联免疫吸附测定（MAC-ELISA）。NS1ICT的敏感性和特异性分别为89.3％和71.4％，IgMICT的敏感性和特异性分别为88％和64.5％。由于抗原交叉反应，报告了假阳性病例。患者的血小板计数与NS1和IgM的ELISA光密度（OD）值相关。在本研究中,血小板计数低的患者显示出高OD值.
■在严重血小板减少症（血小板计数<50000）的情况下，通过ICT筛查和ELISA（NS1和IgM）确认的早期诊断将减少DHF和DSS等并发症。
■在三级护理医院（TCH），研究了2021年2月至2022年2月可能登革热病例的社会人口统计学状况.登革热抗原NS1和IgMICT的结果，登革热NS1Microlisa,比较了登革热IgMMicrolisa，以确定有效管理患者和预防DHF和DSS等并发症的方法。
■在100个ICT反应样品的分布中，50%为NS1反应性,50%为IgM反应性。进一步处理了100个ICT反应样品，用于NS1和IgM的IgM抗体捕获酶联免疫吸附测定（MAC-ELISA）。NS1ICT的敏感性和特异性分别为89.3％和71.4％，IgMICT的敏感性和特异性分别为88％和64.5％。由于抗原交叉反应，报告了假阳性病例。患者的血小板计数与NS1和IgM的ELISA光密度（OD）值相关。在本研究中,血小板计数低的患者显示出高OD值.
■在严重血小板减少症（血小板计数<50000）的情况下，通过ICT筛查和ELISA（NS1和IgM）确认的早期诊断将减少DHF和DSS等并发症。
