PDF(Pubmed)
Abstract：
The objective of this study was to examine the effects of supplemental β-alanine feeding on the athletic performance of Yili horses involved in speed racing, focusing on alterations in plasma free amino acid patterns pre and post exercise. Additionally, the research aimed to evaluate the effects of carnosine on the plasma acid-base buffering capacity and antioxidant levels in these horses. Twelve Yili horse stallions, averaging 3 years in age and 346.50 ± 21.39 kg in weight, were chosen and randomly divided into two groups: a control group and a test group, each comprising six horses. The control group received a supplementation of 300 mg/kg BW/day of α-alanine, while the test group received 300 mg/kg BW/day of β-alanine. This supplementation regimen was maintained for a 30-day supplementation trial period, under identical feeding and management conditions. Throughout the trial, the horses participated in the 1,000 Speed Race, and three distinct blood samples were gathered for assessing plasma free amino acids, blood gases, biochemical parameters, and antioxidant parameters. The outcomes indicated a considerable enhancement in the 1,000 m exercise performance of the speed racing Yili horses in the test group compared to the control group, showcasing a noteworthy improvement of 12.01%, with the test group completing the race 13.29 s faster. Notably, the α-alanine content in the plasma of the control group Yili horses remained higher than that of the test group, demonstrating a consistent increasing trend. By contrast, the plasma β-alanine content was notably higher in the test group than in the control group. Over the course of the supplementation period, plasma β-alanine exhibited an escalating and then stabilizing trend in the test group, whereas in the control group, although β-alanine content also increased, the trend was less pronounced. The plasma levels of histidine and carnosine showed minimal variance between the two groups. Overall, the test group of Yili horses exhibited slightly higher plasma levels of histidine and carnosine compared to the control group. The addition of β-alanine to their diet for a duration of 30 days notably affected the plasma levels of amino acids both pre- and post-exercise in speed-racing Yili horses. Furthermore, β-alanine demonstrated an inhibitory effect on the catabolism of these horses\' bodies during high-intensity exercise. Ten marker amino acids, including valine, leucine, β-alanine, isoleucine, carnosine, 3-methyl-histidine, lysine, ethanolamine, argnine, and taurine, displayed statistically significant changes. β-alanine notably increased the blood glucose levels of Yili horses and played a role in expediting the restoration of blood gas levels post-exercise. Moreover, in the test group of Yili horses, the levels of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity significantly increased both before and after the race, while the content of malondialdehyde, an oxidation product, exhibited an extremely significant decrease immediately after the race. These outcomes suggest that the addition of β-alanine significantly augmented antioxidant levels during high-intensity exercise in Yili horses. Consequently, it reduced post-exercise injuries and accelerated the recovery process after exercise.
摘要：
这项研究的目的是研究补充β-丙氨酸喂养对参与速度赛马的伊犁马运动表现的影响，关注运动前后血浆游离氨基酸模式的变化。此外,该研究旨在评估肌肽对这些马的血浆酸碱缓冲能力和抗氧化剂水平的影响。十二匹伊犁马，平均3岁，体重346.50±21.39公斤，选择并随机分为两组：对照组和测试组，每个人都有六匹马。对照组接受300毫克/千克体重/天的α-丙氨酸，而试验组接受300mg/kgBW/天的β-丙氨酸。这种补充方案维持了30天的补充试验期，在相同的喂养和管理条件下。在整个审判过程中,马匹参加了1000速成赛,收集了三个不同的血液样本来评估血浆游离氨基酸，血气,生化参数，和抗氧化剂参数。结果表明，与对照组相比，测试组速度赛马伊利马的1,000m运动性能有了显着增强，表现出12.01%的显著改善，测试组以13.29秒的速度完成比赛。值得注意的是,对照组伊犁马血浆中的α-丙氨酸含量仍然高于试验组，显示出持续增加的趋势。相比之下,试验组血浆β-丙氨酸含量明显高于对照组。在补充期间，血浆β-丙氨酸在试验组中表现出逐渐升高然后稳定的趋势，而在对照组中，虽然β-丙氨酸含量也增加了，趋势不太明显。组氨酸和肌肽的血浆水平在两组之间显示最小差异。总的来说,与对照组相比，伊犁马的测试组显示出略高的组氨酸和肌肽血浆水平。在30天的饮食中添加β-丙氨酸显着影响了速度赛马伊犁马运动前后的血浆氨基酸水平。此外，在高强度运动过程中，β-丙氨酸对这些马体的分解代谢具有抑制作用。十个标记氨基酸，包括缬氨酸,亮氨酸,β-丙氨酸,异亮氨酸,肌肽,3-甲基组氨酸,赖氨酸，乙醇胺,阿格宁，和牛磺酸,显示出统计学上显著的变化。β-丙氨酸显着增加了伊犁马的血糖水平，并在加快运动后血气水平的恢复中发挥作用。此外，在伊犁马的测试组中，超氧化物歧化酶的水平，谷胱甘肽过氧化物酶,总抗氧化能力在比赛前后都显著增加，而丙二醛的含量,氧化产物，比赛结束后立即表现出极其显著的下降。这些结果表明，在伊犁马进行高强度运动时，添加β-丙氨酸可显着提高抗氧化剂水平。因此,它减少了运动后的伤害，加速了运动后的恢复过程。
