PDF(Pubmed)
Abstract：
There has been a growing interest in skin beauty and antimelanogenic products. Melanogenesis is the process of melanin synthesis whereby melanocytes are activated by UV light or hormone stimulation to produce melanin. Melanogenesis is mediated by several enzymes, such as tyrosinase (TYR), microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1), and TRP-2. In this study, we investigated the effect of Tuber himalayense extract on melanin synthesis in α-melanocyte-stimulating hormone (α-MSH)-treated B16F10 melanoma cells. We confirmed that T. himalayense extract was not toxic to α-MSH-treated B16F10 melanoma cells and exhibited a significant inhibitory effect on melanin synthesis at concentrations of 25, 50, and 100 μg/ml. Additionally, the T. himalayense extract inhibited melanin, TRP-1, TRP-2, tyrosinase, and MITF, which are enzymes involved in melanin synthesis, in a concentration-dependent manner. Furthermore, T. himalayense extract inhibited the mitogen-activated protein kinase (MAPK) pathways, such as extracellular signal-regulated kinase-1/2 (ERK), c-Jun N-terminal kinase (JNK), and p38. Therefore, we hypothesized that various components of T. himalayense extract affect multiple factors involved in melanogenesis in B16F10 cells. Our results indicate that T. himalayense extract could potentially be used as a new material for preparing whitening cosmetics.
摘要：
人们对皮肤美容和抗皮肤生成产品的兴趣与日俱增。黑色素生成是黑色素合成的过程,其中黑色素细胞被UV光或激素刺激激活以产生黑色素。黑色素生成是由几种酶介导的，如酪氨酸酶（TYR），小眼症相关转录因子(MITF),酪氨酸酶相关蛋白-1（TRP-1），和TRP-2。在这项研究中,我们研究了块茎喜马拉雅提取物对α-黑素细胞刺激激素（α-MSH）处理的B16F10黑色素瘤细胞黑色素合成的影响。我们证实，喜马拉雅提取物对α-MSH处理的B16F10黑色素瘤细胞没有毒性，并且在25、50和100μg/ml的浓度下对黑色素合成具有显着的抑制作用。此外,喜马拉雅提取物抑制黑色素，TRP-1,TRP-2,酪氨酸酶,还有MITF,它们是黑色素合成中的酶，以浓度依赖的方式。此外，喜马拉雅提取物抑制丝裂原活化蛋白激酶（MAPK）途径，如细胞外信号调节激酶-1/2（ERK），c-Jun氨基末端激酶（JNK），p38因此,我们假设喜马拉雅提物的各种成分会影响B16F10细胞黑素生成的多种因素。我们的结果表明，喜马拉雅提取物有可能作为制备美白化妆品的新材料。
