PDF(Pubmed)
Abstract：
Trichomonas gallinae (T. gallinae) is a globally distributed protozoan parasite and could cause serious damage to the pigeon industry. MiRNAs have important roles in regulating parasite infection, but its impacts on T. gallinae resistance have rarely been reported. In the present study, we identified a new miRNA (novel-miR-741) and its predicted target OTU deubiquitinase 1 (OTUD1) that might be associated with immunity to T. gallinae in pigeon. Novel-miR-741 and OTUD1 over-expression vectors and interference vectors were constructed. Results from dual luciferase activity assay demonstrated that OTUD1 was a downstream target of novel-miR-741. The Cell Counting Kit-8 and apoptosis assays showed that novel-miR-741 inhibited the proliferation and promoted apoptosis of pigeon crop fibroblasts. Meanwhile, mRNA levels of OTUD1 were significantly reduced in novel-miR-741 mimic-transfected fibroblasts, while mRNA levels of OTUD1 were significantly increased in the novel-miR-741 inhibitor-transfected fibroblasts. The regulatory roles of si-OTUD1 on fibroblasts proliferation, apoptosis, and migration were similar to novel-miR-741 mimic. Our findings demonstrated that novel-miR-741 inhibited the proliferation, and migration of crop fibroblasts, while OTUD1 promoted the proliferation and migration of crop fibroblasts. Therefore, the regulation of OTUD1 by novel-miR-741 was proposed as a potential therapeutic strategy for T. gallinae.
摘要：
鸡毛滴虫(T.gallinae）是全球分布的原生动物寄生虫，可能对鸽子业造成严重破坏。miRNAs在调节寄生虫感染中具有重要作用,但其对鸡粪耐药性的影响鲜有报道。在本研究中,我们鉴定了一种新的miRNA(new-miR-741)及其预测的靶OTU去泛素酶1(OTUD1),它们可能与鸽子对鸡胆虫的免疫相关.构建了new-miR-741和OTUD1过表达载体和干扰载体。来自双荧光素酶活性测定的结果表明OTUD1是新miR-741的下游靶标。细胞计数试剂盒-8和细胞凋亡检测显示，new-miR-741抑制了鸽子作物成纤维细胞的增殖并促进了细胞凋亡。同时,在new-miR-741模拟转染的成纤维细胞中，OTUD1的mRNA水平显着降低，而在新miR-741抑制剂转染的成纤维细胞中,OTUD1的mRNA水平显著升高.si-OTUD1对成纤维细胞增殖的调节作用，凋亡，和迁移类似于新的miR-741模拟物。我们的发现表明，新的miR-741抑制增殖，和作物成纤维细胞的迁移，而OTUD1促进作物成纤维细胞的增殖和迁移。因此,new-miR-741对OTUD1的调节被认为是一种潜在的治疗方案.
