PDF(Pubmed)
Abstract：
The use of mammalian models for in vivo testing of bacterial virulence raises ethical concerns and is expensive and time-consuming. As an alternative, non-mammalian models are sought. Galleria mellonella larvae have been used as a model to study several bacterial pathogens. However, their maintenance is challenging, and commercial supply is low. In this study, we aimed to establish the Zophobas morio larvae as an alternative non-mammalian model for the evaluation of the pathogenicity and antimicrobial susceptibility of Acinetobacter baumannii. We infected Z. morio with Acinetobacter strains and determined the optimal temperature and inoculum. To visualize the bacterial distribution within the larvae, hematoxylin and eosin (H&E) staining was performed. Next, a survival model of infected larvae was established, and virulence was compared between strains. The effect of antimicrobial treatment in relation to antibiotic susceptibility was studied. Our results demonstrate that Z. morio can be used as a model system for in vivo studies of A. baumannii.
摘要：
使用哺乳动物模型进行体内细菌毒力测试引起了伦理问题,并且是昂贵且耗时的。作为替代,寻找非哺乳动物模型。云形藻幼虫已被用作研究几种细菌病原体的模型。然而,他们的维护是具有挑战性的，商业供应低。在这项研究中,我们旨在建立Zophobasmorio幼虫作为一种替代的非哺乳动物模型，用于评估鲍曼不动杆菌的致病性和抗菌药物敏感性。我们用不动杆菌菌株感染了Z.morio，并确定了最佳温度和接种物。为了可视化幼虫内的细菌分布，进行苏木精和伊红(H&E)染色。接下来,建立了感染幼虫的存活模型，并在菌株之间比较毒力。研究了抗菌药物治疗与抗生素敏感性的关系。我们的结果表明，Z.morio可以用作鲍曼不动杆菌体内研究的模型系统。
