METHODS: We used a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach to investigate metabolites associated with gut metabolism in paediatric participants by analysing the urine samples collected from a control group (n = 40), and an experimental group (n = 35) with confirmed TBM, which were subdivided into TBM stage 1 (n = 8), stage 2 (n = 11) and stage 3 (n = 16).
RESULTS: Our metabolomics investigation showed that, of the 78 initially selected compounds of microbiome origin, eight unique urinary metabolites were identified: 2-methylbutyrlglycine, 3-hydroxypropionic acid, 3-methylcrotonylglycine, 4-hydroxyhippuric acid, 5-hydroxyindoleacetic acid, 5-hydroxyhexanoic acid, isobutyrylglycine, and phenylacetylglutamine as urinary markers of dysbiosis in TBM.
CONCLUSIONS: These results - which are supported by previous urinary studies of tuberculosis - highlight the importance of gut metabolism and of identifying corresponding microbial metabolites as novel points for the foundation of improved management of TBM patients.
方法:我们使用靶向液相色谱-串联质谱(LC-MS/MS)方法,通过分析从对照组(n=40)收集的尿液样本,研究儿科参与者与肠道代谢相关的代谢物。和一个实验组(n=35)确认TBM,细分为TBM阶段1(n=8),阶段2(n=11)和阶段3(n=16)。
结果:我们的代谢组学研究表明,在最初选择的78种微生物组来源的化合物中,确定了八种独特的尿代谢物:2-甲基丁酰甘氨酸,3-羟基丙酸,3-甲基巴豆酰基甘氨酸,4-羟基马尿酸,5-羟基吲哚乙酸,5-羟基己酸,异丁酰基甘氨酸,苯乙酰谷氨酰胺作为TBM中生态失调的尿标志物。
结论:这些结果得到了先前关于结核病的泌尿系统研究的支持,突出了肠道代谢和确定相应的微生物代谢产物作为改善TBM患者管理基础的新观点的重要性。