Abstract：
Fusarium wilt, caused by the soilborne fungus Fusarium oxysporum f. sp. vasinfectum (FOV), is a devastating disease affecting cotton (Gossypium spp.) worldwide. Understanding the genetic basis of resistance in diploid cotton and successfully transferring the resistance to tetraploid Upland cotton (G. hirsutum) are crucial for developing resistant cotton cultivars. Although numerous studies have been conducted to investigate the genetic basis of Fusarium wilt in tetraploid cotton, little research has been conducted on diploid species. In this study, an association mapping panel consisting of 246 accessions of G. arboreum, was used to identify chromosomal regions for FOV race 4 (FOV4) resistance based on foliar disease severity ratings in four greenhouse tests. Through a genome-wide association study (GWAS) based on 7,009 single nucleotide polymorphic (SNP) markers, 24 FOV4 resistance QTLs, including three major QTLs on chromosomes A04, A06, and A11, were detected. A validation panel consisting of 97 diploid cotton accessions was employed, confirming the presence of several QTLs. Evaluation of an introgressed BC2F7 population derived from G. hirsutum/G. aridum/G. arboreum showed significant differences in disease incidence and mortality rate, as compared to susceptible and resistant controls, suggesting that the resistance in G. arboreum and/or G. aridum was transferred into Upland cotton for the first time. The identification of novel major resistance QTLs, along with the transfer of resistance from the diploid species, expands our understanding of the genomic regions involved in conferring resistance to FOV4 and contributes to the development of resilient Upland cotton cultivars.
摘要：
枯萎病,由土壤传播的真菌尖孢镰刀菌引起。血管感染（FOV），是一种影响棉花的毁灭性疾病（棉属。）全世界。了解二倍体棉花抗性的遗传基础，并成功地将抗性转移到四倍体陆地棉（G。hirsutum）对于开发抗性棉花品种至关重要。尽管已经进行了许多研究来调查四倍体棉花枯萎病的遗传基础，关于二倍体物种的研究很少。在这项研究中,一个由246个G.arboreum种质组成的协会映射小组，根据四个温室测试中的叶面疾病严重程度等级，用于鉴定FOV种族4（FOV4）抗性的染色体区域。通过基于7,009个单核苷酸多态性（SNP）标记的全基因组关联研究（GWAS），24个FOV4电阻QTL，在染色体A04、A06和A11上检测到三个主要QTL。采用了由97个二倍体棉花种质组成的验证小组，确认存在多个QTL。对源自G.hirsutum/G的渗入BC2F7群体的评估aridum/G.植物园在疾病发病率和死亡率方面表现出显著差异，与易感和抗性对照相比，表明G.arboreum和/或G.aridum的抗性首次转移到陆地棉中。新型主要抗性QTLs的鉴定，随着抗性从二倍体物种的转移，扩大了我们对赋予FOV4抗性的基因组区域的理解，并有助于有弹性的陆地棉品种的发展。
