PDF(Pubmed)
Abstract：
The aim of this study was to develop an efficient and accurate platform for the detection of the newly identified goose megrivirus (GoMV). To achieve this goal, we developed a TaqMan real-time PCR technology for the rapid detection and identification of GoMV. Our data showed that the established TaqMan real-time PCR assay had high sensitivity, with the lowest detection limit of 67.3 copies/μL. No positive signal can be observed from other goose origin viruses (including AIV, GPV, GoCV, GHPyV, and GoAstV), with strong specificity. The coefficients of variation of repeated intragroup and intergroup tests were all less than 1.5%, with excellent repeatability. Clinical sample investigation data from domestic Minbei White geese firstly provided evidence that GoMV can be transmitted both horizontally and vertically. In conclusion, since the TaqMan real-time PCR method has high sensitivity, specificity, and reproducibility, it can be a useful candidate tool for GoMV epidemiological investigation.
摘要：
本研究的目的是开发一种高效，准确的平台，用于检测新鉴定的鹅巨病毒（GoMV）。为了实现这一目标,我们开发了TaqMan实时PCR技术，用于快速检测和鉴定GoMV。我们的数据表明，建立的TaqMan实时PCR检测方法具有较高的灵敏度，最低检测限为67.3拷贝/μL。从其他鹅源病毒（包括AIV，GPV,GoCV,GHPyV,和GoAstV），具有很强的特异性。重复组内和组间试验的变异系数均小于1.5%,具有良好的可重复性。来自国内闽北白鹅的临床样本调查数据首次提供了GoMV可以水平和垂直传播的证据。总之,由于TaqMan实时PCR方法具有较高的灵敏度,特异性，和再现性，它可以成为GoMV流行病学调查的有用候选工具。
