PDF(Pubmed)
Abstract：
UNASSIGNED: The purpose of this study was to investigate rod photopigment bleaching-driven intrinsic optical signals (IOS) in the human outer retina and its measurement repeatability based on a commercial optical coherence tomography (OCT) platform.
UNASSIGNED: The optical path length of the rod photoreceptor subretinal space (SRS), that is, the distance between signal bands of rod outer segment tips and retinal pigment epithelium, was measured in 15 healthy subjects in ambient light and during a long-duration bleaching white-light exposure.
UNASSIGNED: On 2 identical study days (day 1 and day 2 [D1 and D2]), light stimulation resulted in a significant decrease in rod SRS by 21.3 ± 7.6% and 19.8 ± 8.5% (both P < 0.001), respectively. The test-retest reliability of the SRS maximum change of an individual subject was moderate for single measures (intraclass correlation coefficient [ICC] = 0.730, 95% confidence interval [CI] = 0.376, 0.900, P < 0.001) and good for average measures (ICC = 0.844, 95% CI = 0.546, 0.947, P < 0.001). The mean area under the stimulus response curve with values of 14.8 ± 9.4 and 15.5 ± 7.5 µm × minutes (P = 0.782) showed excellent agreement between the stimulus response on D1 and D2. Intermittent dark adaptation of the retina led to an initial increase of the SRS by 6.1% (P = 0.018) and thereafter showed a decrease toward baseline, despite continued dark adaptation.
UNASSIGNED: The data indicate the potential of commercial OCT in measuring slow IOS in the outer retina suggesting that the rod SRS could serve as a biomarker for photoreceptor function. The presented approach could provide an easily implementable clinical tool for the early detection of diseases affecting photoreceptor health.
UNASSIGNED: The optical path length of the rod photoreceptor subretinal space (SRS), that is, the distance between signal bands of rod outer segment tips and retinal pigment epithelium, was measured in 15 healthy subjects in ambient light and during a long-duration bleaching white-light exposure.
UNASSIGNED: On 2 identical study days (day 1 and day 2 [D1 and D2]), light stimulation resulted in a significant decrease in rod SRS by 21.3 ± 7.6% and 19.8 ± 8.5% (both P < 0.001), respectively. The test-retest reliability of the SRS maximum change of an individual subject was moderate for single measures (intraclass correlation coefficient [ICC] = 0.730, 95% confidence interval [CI] = 0.376, 0.900, P < 0.001) and good for average measures (ICC = 0.844, 95% CI = 0.546, 0.947, P < 0.001). The mean area under the stimulus response curve with values of 14.8 ± 9.4 and 15.5 ± 7.5 µm × minutes (P = 0.782) showed excellent agreement between the stimulus response on D1 and D2. Intermittent dark adaptation of the retina led to an initial increase of the SRS by 6.1% (P = 0.018) and thereafter showed a decrease toward baseline, despite continued dark adaptation.
UNASSIGNED: The data indicate the potential of commercial OCT in measuring slow IOS in the outer retina suggesting that the rod SRS could serve as a biomarker for photoreceptor function. The presented approach could provide an easily implementable clinical tool for the early detection of diseases affecting photoreceptor health.
摘要：
■这项研究的目的是基于商业光学相干断层扫描（OCT）平台研究人类外视网膜中的杆状光色素漂白驱动的固有光信号（IOS）及其测量可重复性。
■杆状感光体视网膜下空间（SRS）的光路长度，也就是说,杆外段尖端信号带与视网膜色素上皮之间的距离，在15名健康受试者中，在环境光和长时间的漂白白光暴露中进行了测量。
■在2个相同的研究日（第1天和第2天[D1和D2]），光刺激导致杆SRS显着下降21.3±7.6％和19.8±8.5％（均P<0.001），分别。单个受试者的SRS最大变化的测试重测可靠性对于单个测量是中等的(组内相关系数[ICC]=0.730,95%置信区间[CI]=0.376,0.900,P<0.001),对于平均测量是良好的(ICC=0.844,95%CI=0.546,0.947,P<0.001)。刺激响应曲线下的平均面积为14.8±9.4和15.5±7.5µm×分钟（P=0.782），在D1和D2上的刺激响应之间显示出极好的一致性。视网膜的间歇性暗适应导致SRS最初增加6.1％（P=0.018），此后显示出基线下降，尽管继续黑暗适应。
■数据表明商业OCT在测量外视网膜中缓慢的IOS方面的潜力，表明杆SRS可以用作光感受器功能的生物标志物。所提出的方法可以为早期检测影响光感受器健康的疾病提供容易实施的临床工具。
■杆状感光体视网膜下空间（SRS）的光路长度，也就是说,杆外段尖端信号带与视网膜色素上皮之间的距离，在15名健康受试者中，在环境光和长时间的漂白白光暴露中进行了测量。
■在2个相同的研究日（第1天和第2天[D1和D2]），光刺激导致杆SRS显着下降21.3±7.6％和19.8±8.5％（均P<0.001），分别。单个受试者的SRS最大变化的测试重测可靠性对于单个测量是中等的(组内相关系数[ICC]=0.730,95%置信区间[CI]=0.376,0.900,P<0.001),对于平均测量是良好的(ICC=0.844,95%CI=0.546,0.947,P<0.001)。刺激响应曲线下的平均面积为14.8±9.4和15.5±7.5µm×分钟（P=0.782），在D1和D2上的刺激响应之间显示出极好的一致性。视网膜的间歇性暗适应导致SRS最初增加6.1％（P=0.018），此后显示出基线下降，尽管继续黑暗适应。
■数据表明商业OCT在测量外视网膜中缓慢的IOS方面的潜力，表明杆SRS可以用作光感受器功能的生物标志物。所提出的方法可以为早期检测影响光感受器健康的疾病提供容易实施的临床工具。
