Abstract：
Apoptosis-inducing factor 1 (AIF1) overexpression is intimately linked to the sensitivity of yeast cells towards hydrogen peroxide or acetic acid. Therefore, studying the mechanism of AIF1 regulation in the cell would provide a significant understanding of the factors guiding yeast apoptosis. In this report, we show the time-dependent induction of AIF1 under hydrogen peroxide stress. Additionally, we find that AIF1 expression in response to hydrogen peroxide is mediated by two transcription factors, Yap5 (DNA binding) and Cdc73 (non-DNA binding). Furthermore, substituting the H3K36 residue with another amino acid significantly abrogates AIF1 expression. However, substituting the lysine (K) in H3K4 or H3K79 with alanine (A) does not affect AIF1 expression level under hydrogen peroxide stress. Altogether, reduced AIF1 expression in cdc73Δ is plausibly due to reduced H3K36me3 levels in the cells.
摘要：
凋亡诱导因子1（AIF1）的过表达与酵母细胞对过氧化氢或乙酸的敏感性密切相关。因此,研究细胞中AIF1调节的机制将为指导酵母凋亡的因素提供重要的理解。在这份报告中,我们显示了过氧化氢胁迫下AIF1的时间依赖性诱导。此外,我们发现AIF1响应过氧化氢的表达是由两个转录因子介导的，Yap5(DNA结合)和Cdc73(非DNA结合)。此外，用另一个氨基酸取代H3K36残基显著地消除AIF1表达。然而,在过氧化氢胁迫下，用丙氨酸（A）代替H3K4或H3K79中的赖氨酸（K）不会影响AIF1的表达水平。总之,cdc73Δ中AIF1表达的减少似乎是由于细胞中H3K36me3水平的降低。
