Abstract：
The interaction between fluorescently labeled hyaluronan and cationic surfactants was studied using Fluorescence Correlation Spectroscopy. The hyaluronan was selected at two different molecular weights - specifically, 274 kDa and 710 kDa. Cetyltrimethylammonium bromide and Septonex® were chosen as cationic surfactants to interact with the negatively charged biopolymer. The study focused on changes in the diffusive behavior of a biopolymer that interacts with surfactant molecules in an aqueous environment. Various methods were applied to evaluate the obtained data, these including, among others, the Maximum Entropy Method, which provides the distributional dependences of diffusion coefficients. Without the surfactant, the studied biopolymers showed diffusion behavior comparable to that found in previously published studies. In the presence of surfactants, more intense interaction was observed between Cetyltrimethylammonium bromide and Septonex®. Comparing the molecular weights, the retention of intermolecular aggregates after the precipitation region for the lower weight and the disintegration of these aggregates for the higher weight were observed; moreover, they showed diffusion behavior comparable to the samples without the presence of the surfactant.
摘要：
使用荧光相关光谱法研究了荧光标记的透明质酸与阳离子表面活性剂之间的相互作用。选择两种不同分子量的透明质酸-特别是，274kDa和710kDa。选择十六烷基三甲基溴化铵和Septonex®作为阳离子表面活性剂以与带负电荷的生物聚合物相互作用。该研究的重点是在水性环境中与表面活性剂分子相互作用的生物聚合物的扩散行为的变化。应用各种方法对获得的数据进行评估，这些包括,其中,最大熵方法，它提供了扩散系数的分布依赖性。没有表面活性剂，所研究的生物聚合物显示出与先前发表的研究相当的扩散行为。在表面活性剂的存在下,在十六烷基三甲基溴化铵和Septonex®之间观察到更强烈的相互作用。比较分子量，观察到较低重量的沉淀区域后分子间聚集体的保留和较高重量的这些聚集体的崩解；此外，它们显示出与不存在表面活性剂的样品相当的扩散行为。
