Abstract：
When the studies are evaluated, immunomodulatory effect of MSCs, administration in critically ill patients, obstacle situations in use and side effects, pulmonary fibrosis prevention, which stem cells and their products, regeneration effect, administration route, and dosage are listed under the main heading like. The effect of MSC administration on DNA repair genes in COVID-19 infection is unknown. Our aim is to determine the effect of mesenchymal stem cells (MSCs) therapy applied in critically ill patients with coronavirus infection on DNA repair pathways and genes associated with those pathways. Patients (n = 30) divided into two equal groups. Group-1: Patients in a critically ill condition, Group-2: Patients in critically ill condition and transplanted MSCs. The mechanism was investigated in eleven genes of five different pathways; Base excision repair: PARP1, Nucleotide excision repair (NER): RAD23B and ERCC1, Homologous recombinational repair (HR): ATM, RAD51, RAD52 and WRN, Mismatch repair (MMR): MLH1, MSH2, and MSH6, Direct reversal repair pathway: MGMT. It was found that MSCs application had a significant effect on 6 genes located in 3 different DNA damage response pathways. These are NER pathway genes; RAD23 and ERCC1, HR pathway genes; ATM and RAD51, MMR pathway genes; MSH2 and MSH6 (p < 0.05). Two main points were shown. First, as a result of cellular damage in critical patients with COVID-19, DNA damage occurs and then DNA repair pathways and genes are activated in reaction to this situation. Second, administration of MSC to patients with COVID-19 infection plays a positive role by increasing the expression of DNA repair genes located in DNA damage pathways.
摘要：
当研究被评估时，MSCs的免疫调节作用，危重病人的管理，使用障碍情况和副作用，肺纤维化预防，哪些干细胞和它们的产品，再生效果,给药途径,和剂量列在主标题下。MSC给药对COVID-19感染中DNA修复基因的影响尚不清楚。我们的目的是确定间充质干细胞（MSCs）治疗应用于冠状病毒感染的危重患者对DNA修复途径和与这些途径相关的基因的影响。患者（n=30）分为两组。第1组：处于危重状态的患者，第2组：危重患者和移植的MSCs。在五种不同途径的11个基因中研究了该机制；碱基切除修复：PARP1，核苷酸切除修复（NER）：RAD23B和ERCC1，同源重组修复（HR）：ATM，RAD51、RAD52和WRN,错配修复(MMR):MLH1、MSH2和MSH6,直接逆转修复途径:MGMT。发现MSCs的应用对位于3个不同DNA损伤应答通路的6个基因有显著的影响。这些是NER途径基因;RAD23和ERCC1,HR途径基因;ATM和RAD51,MMR途径基因;MSH2和MSH6(p<0.05)。展示了两个要点。首先,由于COVID-19危重患者的细胞损伤，会发生DNA损伤，然后DNA修复途径和基因被激活。第二,向COVID-19感染患者施用MSC通过增加位于DNA损伤途径中的DNA修复基因的表达发挥积极作用。
