Abstract：
Adult T-cell leukemia/lymphoma (ATL), caused by human T-cell leukemia virus type-1 (HTLV-1) infection, is a malignant hematologic cancer that remains difficult to cure. We herein established a biomarker identification strategy based on the total cell proteomics of cultured ATL cells to search for novel ATL biomarkers. Four protocols with a combination of selected conditions based on lysis buffers and addition agents for total cell proteomics were used for a differential analysis between the ATL cell group (consisting of 11 cell lines), HTLV-1-infected cell group (consisting of 6 cell lines), and HTLV-1-negative cell group (consisting of 6 cell lines). In the analysis, we identified 24 and 27 proteins that were significantly increased (ratio ≥2.0, p < 0.05) and decreased (ratio ≤ 0.5, p < 0.05), respectively, in the ATL group. Previously reported CCL3 and CD30/TNFRSF8 were confirmed to be among significantly increased proteins. Furthermore, correlation analysis between identified proteins and Tax suggested that RASSF2 and GORASP2 were candidates of novel Tax-regulated factors. The biomarker identification strategy established herein is expected to contribute to the identification of biomarkers for ATL and other diseases.
摘要：
成人T细胞白血病/淋巴瘤(ATL),由人类T细胞白血病病毒1型（HTLV-1）感染引起，是一种难以治愈的恶性血液肿瘤。我们在此建立了基于培养的ATL细胞的总细胞蛋白质组学的生物标志物鉴定策略,以寻找新的ATL生物标志物。使用基于裂解缓冲液和用于总细胞蛋白质组学的添加剂的选定条件的组合的四种方案用于ATL细胞组（由11种细胞系组成）之间的差异分析。HTLV-1感染的细胞组（由6个细胞系组成），和HTLV-1阴性细胞组(由6个细胞系组成)。在分析中,我们确定了24和27个蛋白质显着增加（比率≥2.0，p<0.05）和减少（比率≤0.5，p<0.05），分别,在ATL组中。先前报道的CCL3和CD30/TNFRSF8被证实是显著增加的蛋白质之一。此外，鉴定的蛋白质与Tax之间的相关性分析表明，RASSF2和GORASP2是新的税收调节因子的候选因子。本文建立的生物标志物鉴定策略预期有助于鉴定ATL和其他疾病的生物标志物。
