PDF(Pubmed)
Abstract：
UNASSIGNED: Traditional Chinese medicine (TCM) is widely used in humans and animals, which is very important for health. TCM affects the body \'s immunity and changes in intestinal flora. This study was conducted to investigate the effects of dietary Hong-bailanshen (HBLS) supplementation in horses on serum biochemical profile, antioxidant enzymes and gut microbiota.
UNASSIGNED: In this study, five horses were selected. On day 0, 14, 28, blood samples and feces were collected on days 0, 14, and 28 to analyse gut microbiota, serum biochemical and redox indexes.
UNASSIGNED: The results showed that the addition of HBLS to horse diets significantly decreased the level of alanine aminotransferase, alkaline phosphatase, creatine kinase and malondialdehyde (p < 0.05, p < 0.01) and significantly increased the activity of total antioxidant capacity, superoxide dismutase and catalase (p < 0.05, p < 0.01). Compared with day 14, the levels of alanine aminotransferase, alkaline phosphatase and creatine kinase were significantly decreased; however, the level of catalase was significantly increased in the horses continuously fed with HBLS for 28 days (p < 0.05, p < 0.01). Alpha diversity analysis was performed that chao1 (p < 0.05), observed_specicies, faith\'pd and goods_coverage upregulated in the horses fed HBLS. A total of 24 differential genera were detected adding HBLS to diet increased the abundance of Bacillus, Lactobacillaceae, Leuconostocaceae, Christensenellaceae, Peptostreptococcaceae, Faecalibacterium, Erysipelotrichaceae, Pyramidobacter, Sphaerochaeta, WCHB1-25, Bacteria, Oscillospira, and Acetobacteraceae, while reduced Aerococcus, EtOH8, Syntrophomonas, Caulobacter, Bradyrhizobiaceae, W22, Succinivibrionaceae, and Desulfovibrio (p < 0.05, p < 0.01).
UNASSIGNED: Adding HBLS to the diet could be a potentially effective strategy to improve horses\' health.
UNASSIGNED: In this study, five horses were selected. On day 0, 14, 28, blood samples and feces were collected on days 0, 14, and 28 to analyse gut microbiota, serum biochemical and redox indexes.
UNASSIGNED: The results showed that the addition of HBLS to horse diets significantly decreased the level of alanine aminotransferase, alkaline phosphatase, creatine kinase and malondialdehyde (p < 0.05, p < 0.01) and significantly increased the activity of total antioxidant capacity, superoxide dismutase and catalase (p < 0.05, p < 0.01). Compared with day 14, the levels of alanine aminotransferase, alkaline phosphatase and creatine kinase were significantly decreased; however, the level of catalase was significantly increased in the horses continuously fed with HBLS for 28 days (p < 0.05, p < 0.01). Alpha diversity analysis was performed that chao1 (p < 0.05), observed_specicies, faith\'pd and goods_coverage upregulated in the horses fed HBLS. A total of 24 differential genera were detected adding HBLS to diet increased the abundance of Bacillus, Lactobacillaceae, Leuconostocaceae, Christensenellaceae, Peptostreptococcaceae, Faecalibacterium, Erysipelotrichaceae, Pyramidobacter, Sphaerochaeta, WCHB1-25, Bacteria, Oscillospira, and Acetobacteraceae, while reduced Aerococcus, EtOH8, Syntrophomonas, Caulobacter, Bradyrhizobiaceae, W22, Succinivibrionaceae, and Desulfovibrio (p < 0.05, p < 0.01).
UNASSIGNED: Adding HBLS to the diet could be a potentially effective strategy to improve horses\' health.
摘要：
■中药（TCM）广泛用于人类和动物，这对健康非常重要。中医影响机体免疫力和肠道菌群的变化。本研究旨在研究马饮食中添加红百兰神（HBLS）对血清生化特征的影响，抗氧化酶和肠道菌群。
■在这项研究中，选择了五匹马。在第0、14、28天，在第0、14和28天收集血液样本和粪便以分析肠道微生物群。血清生化和氧化还原指标。
■结果表明，在马的日粮中添加HBLS可显着降低丙氨酸转氨酶的水平，碱性磷酸酶,肌酸激酶和丙二醛（p<0.05，p<0.01），并显着增加了总抗氧化能力的活性，超氧化物歧化酶和过氧化氢酶(p<0.05,p<0.01)。与第14天相比，丙氨酸转氨酶水平，碱性磷酸酶和肌酸激酶显著下降；然而，连续饲喂HBLS28天的马过氧化氢酶水平显着增加（p<0.05，p<0.01）。进行α多样性分析，chao1(p<0.05)，观察到的_规格，信仰\'pd和货物_覆盖率在喂HBLS的马匹中上调。共检测到24个差异属，在饮食中添加HBLS增加了芽孢杆菌的丰度，乳酸杆菌科，明串珠科,Christensenellaceae,肽链球菌科，粪杆菌,Erysipelotricaceae，吡喃杆菌,Sphaerochaeta,WCHB1-25,细菌,螺旋体,和醋杆菌科，虽然减少了空气球菌，EtOH8，合成单胞菌，Caulobacter,根瘤菌科，W22,丁烟菌科,和脱硫弧菌(p<0.05,p<0.01)。
在饮食中添加HBLS可能是改善马健康的潜在有效策略。
■在这项研究中，选择了五匹马。在第0、14、28天，在第0、14和28天收集血液样本和粪便以分析肠道微生物群。血清生化和氧化还原指标。
■结果表明，在马的日粮中添加HBLS可显着降低丙氨酸转氨酶的水平，碱性磷酸酶,肌酸激酶和丙二醛（p<0.05，p<0.01），并显着增加了总抗氧化能力的活性，超氧化物歧化酶和过氧化氢酶(p<0.05,p<0.01)。与第14天相比，丙氨酸转氨酶水平，碱性磷酸酶和肌酸激酶显著下降；然而，连续饲喂HBLS28天的马过氧化氢酶水平显着增加（p<0.05，p<0.01）。进行α多样性分析，chao1(p<0.05)，观察到的_规格，信仰\'pd和货物_覆盖率在喂HBLS的马匹中上调。共检测到24个差异属，在饮食中添加HBLS增加了芽孢杆菌的丰度，乳酸杆菌科，明串珠科,Christensenellaceae,肽链球菌科，粪杆菌,Erysipelotricaceae，吡喃杆菌,Sphaerochaeta,WCHB1-25,细菌,螺旋体,和醋杆菌科，虽然减少了空气球菌，EtOH8，合成单胞菌，Caulobacter,根瘤菌科，W22,丁烟菌科,和脱硫弧菌(p<0.05,p<0.01)。
在饮食中添加HBLS可能是改善马健康的潜在有效策略。
