PDF(Pubmed)
Abstract：
The <3% dissimilar Amplicon Sequence Variant (ASV) clusters of the 18S-V4 barcode were used as species-proxies for the evaluation of ASV composition and ASV diversity indices characterizing the hitherto poorly investigated meiofaunal communities of the south-eastern part of the Levantine basin. Accompanied by abundance measurements, the relationships of these characteristics with sedimentary and bottom terrain parameters were interpreted. The construction of community composition profiles, namely ASVs\' list and their estimated abundances, was done using our previously established procedure (Harbuzov et al., 2022, Marine Genomics 65, 100980), combining metabarcoding with sample reads normalization by the abundance of hard-bodied meiofaunal taxa. The study province included the 54-1418 m depth range, across vertical sub-bottom horizons ranging 0-17 cm. Oxygen, hydrogen sulfide and methane concentrations in the pore water, as well as sediment grain size spectra and sedimentary protein and carbohydrate levels, were measured, followed by an evaluation of their involvement in the shaping of the meiofaunal communities\' characteristics. Community composition was generally site-and-horizon dependent and its abundance decreased with increasing bottom depth and across sub-bottom horizons, typical to benthic habitats which are nourished by organic carbon from the euphotic zone. The relatively sharply inclined continental slope bottom located in the northern part of the Israeli coast was an exception. Its meiofaunal community characteristics were speculated to be affected by intensive sediment mixing and lateral transport of food from the shelf, in addition to the effect of the euphotic zone-originated food sources.
摘要：
18S-V4条形码的<3％不同的扩增子序列变异体（ASV）簇被用作物种代理，用于评估ASV组成和ASV多样性指数，这些指数表征了迄今为止对东南部未充分调查的小型群落的特征。黎凡特盆地。伴随着丰度测量，解释了这些特征与沉积和底部地形参数的关系。社区构成概况的构建，即ASV列表及其估计丰度，是使用我们以前建立的程序完成的(Harbuzov等人。,2022,MarineGenomics65,100980),通过丰富的硬体meiofaunal分类群，将元编码与样本读数归一化相结合。研究省包括54-1418米的深度范围，跨越垂直底部的水平范围为0-17厘米。氧气,孔隙水中的硫化氢和甲烷浓度，以及沉积物粒度谱和沉积蛋白质和碳水化合物水平，被测量，其次是对他们参与塑造meiofaunal社区特征的评估。群落组成通常取决于地点和地平线，其丰度随着底部深度和底部地层的增加而减少，典型的底栖栖息地，这些栖息地由富营养带的有机碳营养。位于以色列海岸北部的相对急剧倾斜的大陆坡底部是一个例外。据推测，其深层群落特征受到强烈的沉积物混合和从架子上横向运输食物的影响，除了来自富营养区的食物来源的影响。
