PDF(Pubmed)
Abstract：
Aim: To explore the overall methylation changes in liver tissues during the formation of gallstones, as well as the key pathways and genes involved in the process. Methods: Reduced-representation bisulfite sequencing and RNA sequencing were conducted on the liver tissues of mice with gallstones and control normal mice. Results: A total of 8705 differentially methylated regions in CpG and 1410 differentially expressed genes were identified. The joint analysis indicated that aberrant DNA methylation may be associated with dysregulated gene expression in key pathways such as cholesterol metabolism and bile secretion. Conclusion: We propose for the first time that methylation changes in some key pathway genes in liver tissue may be involved in the formation of gallstones.
摘要：
目的:探讨胆结石形成过程中肝组织的整体甲基化变化,以及参与该过程的关键途径和基因。方法：对胆结石小鼠和对照正常小鼠的肝组织进行减少代表亚硫酸氢盐测序和RNA测序。结果：共鉴定出CpG中8705个差异甲基化区域和1410个差异表达基因。联合分析表明，异常的DNA甲基化可能与胆固醇代谢和胆汁分泌等关键途径中的基因表达失调有关。结论:我们首次提出肝组织中一些关键通路基因的甲基化改变可能参与了胆结石的形成。
