PDF(Pubmed)
Abstract：
Dietary patterns and corresponding gut microbiota profiles are associated with various health conditions. A diet rich in polyphenols, primarily plant-based, has been shown to promote the growth of probiotic bacteria in the gastrointestinal tract, subsequently reducing the risk of metabolic disorders in the host. The beneficial effects of these bacteria are largely due to the specific metabolites they produce, such as short-chain fatty acids and membrane proteins. In this study, we employed a metabolomics-guided bioactive metabolite identification platform that included bioactivity testing using in vitro and in vivo assays to discover a bioactive metabolite produced from probiotic bacteria. Through this approach, we identified 5\'-methylthioadenosine (MTA) as a probiotic bacterial-derived metabolite with anti-obesity properties. Furthermore, our findings indicate that MTA administration has several regulatory impacts on liver functions, including modulating fatty acid synthesis and glucose metabolism. The present study elucidates the intricate interplay between dietary habits, gut microbiota, and their resultant metabolites.
摘要：
饮食模式和相应的肠道微生物群与各种健康状况相关。富含多酚的饮食，主要是植物性的，已被证明可以促进胃肠道中益生菌的生长，随后降低宿主代谢紊乱的风险。这些细菌的有益作用主要是由于它们产生的特定代谢产物，如短链脂肪酸和膜蛋白。在这项研究中,我们采用了代谢组学指导的生物活性代谢物鉴定平台,该平台包括使用体外和体内试验的生物活性测试,以发现由益生菌产生的生物活性代谢物.通过这种方法，我们确定5'-甲硫腺苷（MTA）是一种具有抗肥胖特性的益生菌代谢产物。此外，我们的研究结果表明，MTA管理对肝功能有几个调节影响，包括调节脂肪酸合成和葡萄糖代谢。本研究阐明了饮食习惯之间复杂的相互作用，肠道菌群，以及它们产生的代谢物。
