PDF(Pubmed)
Abstract：
UNASSIGNED: Oxidative stress is involved in pathogenesis of some psychiatric disorders. To examine the role of oxidative stress in the etiopathogenesis of obsessive-compulsive disorder (OCD), we aimed to determine oxidative stress indices, including malondialdehyde (MDA) levels in serum and red blood cells (RBC) membrane, total antioxidant capacity (TAC), serum glutathione (GSH) levels, serum antioxidant vitamins (A and E), and Na+/K+-ATPase activity, in patients with the mentioned disorder vs. healthy controls.
UNASSIGNED: 39 OCD patients diagnosed based on Diagnostic and Statistical Manual of Mental Disorders (DSM-V) and 39 volunteer healthy subjects were included in this study. MDA levels in serum and RBC membrane were measured using fluorometric method. Serum TAC level, serum GSH level, and Na+/K+-ATPase activity were also measured using spectrophotometric methods. Serum levels of vitamins were calculated by reversed-phase high-performance liquid chromatography (RP-HPLC).
UNASSIGNED: There was a significantly higher MDA level in serum (p < 0.0001) and RBC membrane (p = 0.002) of OCD patients compared with those in controls. A significant reduction in vitamin A (p = 0.001) and vitamin E (p = 0.024) levels was found in OCD patients vs. controls. There was significantly lower activity of erythrocyte membrane Na+/K+-ATPase in RBC membrane of OCD patients vs. controls (p < 0.0001).
UNASSIGNED: Our findings indicate significantly higher levels MDA in both serum and RBC membrane, lower levels of serum vitamins A and E, and lower activity of membrane Na+/K+-ATPase in OCD patients compared to controls. These suggest an imbalance between oxidant and antioxidant factors in OCD patients that might play a fundamental role in the etiopathogenesis of OCD.
UNASSIGNED: 39 OCD patients diagnosed based on Diagnostic and Statistical Manual of Mental Disorders (DSM-V) and 39 volunteer healthy subjects were included in this study. MDA levels in serum and RBC membrane were measured using fluorometric method. Serum TAC level, serum GSH level, and Na+/K+-ATPase activity were also measured using spectrophotometric methods. Serum levels of vitamins were calculated by reversed-phase high-performance liquid chromatography (RP-HPLC).
UNASSIGNED: There was a significantly higher MDA level in serum (p < 0.0001) and RBC membrane (p = 0.002) of OCD patients compared with those in controls. A significant reduction in vitamin A (p = 0.001) and vitamin E (p = 0.024) levels was found in OCD patients vs. controls. There was significantly lower activity of erythrocyte membrane Na+/K+-ATPase in RBC membrane of OCD patients vs. controls (p < 0.0001).
UNASSIGNED: Our findings indicate significantly higher levels MDA in both serum and RBC membrane, lower levels of serum vitamins A and E, and lower activity of membrane Na+/K+-ATPase in OCD patients compared to controls. These suggest an imbalance between oxidant and antioxidant factors in OCD patients that might play a fundamental role in the etiopathogenesis of OCD.
摘要：
■氧化应激与某些精神疾病的发病机制有关。为了研究氧化应激在强迫症（OCD）病因中的作用，我们的目标是确定氧化应激指数，包括血清和红细胞（RBC）膜中丙二醛（MDA）水平，总抗氧化能力(TAC)，血清谷胱甘肽（GSH）水平，血清抗氧化维生素（A和E），和Na+/K+-ATP酶活性，在上述疾病患者中与健康的控制。
■本研究纳入了根据《精神障碍诊断和统计手册》（DSM-V）诊断的39名OCD患者和39名自愿健康受试者。采用荧光法测定血清和红细胞膜中MDA含量。血清TAC水平,血清GSH水平,还使用分光光度法测量了Na/K-ATPase活性。通过反相高效液相色谱法（RP-HPLC）计算血清维生素水平。
■与对照组相比，OCD患者的血清（p<0.0001）和RBC膜（p=0.002）中的MDA水平显着升高。在强迫症患者中发现维生素A（p=0.001）和维生素E（p=0.024）水平显着降低。controls.强迫症患者红细胞膜Na+/K+-ATP酶活性明显低于对照（p<0.0001）。
■我们的发现表明血清和红细胞膜中MDA的水平明显升高，血清维生素A和E水平较低，与对照组相比，OCD患者的膜Na/K-ATPase活性较低。这些表明OCD患者中氧化剂和抗氧化剂因子之间的不平衡，这可能在OCD的病因中起着根本作用。
■本研究纳入了根据《精神障碍诊断和统计手册》（DSM-V）诊断的39名OCD患者和39名自愿健康受试者。采用荧光法测定血清和红细胞膜中MDA含量。血清TAC水平,血清GSH水平,还使用分光光度法测量了Na/K-ATPase活性。通过反相高效液相色谱法（RP-HPLC）计算血清维生素水平。
■与对照组相比，OCD患者的血清（p<0.0001）和RBC膜（p=0.002）中的MDA水平显着升高。在强迫症患者中发现维生素A（p=0.001）和维生素E（p=0.024）水平显着降低。controls.强迫症患者红细胞膜Na+/K+-ATP酶活性明显低于对照（p<0.0001）。
■我们的发现表明血清和红细胞膜中MDA的水平明显升高，血清维生素A和E水平较低，与对照组相比，OCD患者的膜Na/K-ATPase活性较低。这些表明OCD患者中氧化剂和抗氧化剂因子之间的不平衡，这可能在OCD的病因中起着根本作用。
