UNASSIGNED: 39 OCD patients diagnosed based on Diagnostic and Statistical Manual of Mental Disorders (DSM-V) and 39 volunteer healthy subjects were included in this study. MDA levels in serum and RBC membrane were measured using fluorometric method. Serum TAC level, serum GSH level, and Na+/K+-ATPase activity were also measured using spectrophotometric methods. Serum levels of vitamins were calculated by reversed-phase high-performance liquid chromatography (RP-HPLC).
UNASSIGNED: There was a significantly higher MDA level in serum (p < 0.0001) and RBC membrane (p = 0.002) of OCD patients compared with those in controls. A significant reduction in vitamin A (p = 0.001) and vitamin E (p = 0.024) levels was found in OCD patients vs. controls. There was significantly lower activity of erythrocyte membrane Na+/K+-ATPase in RBC membrane of OCD patients vs. controls (p < 0.0001).
UNASSIGNED: Our findings indicate significantly higher levels MDA in both serum and RBC membrane, lower levels of serum vitamins A and E, and lower activity of membrane Na+/K+-ATPase in OCD patients compared to controls. These suggest an imbalance between oxidant and antioxidant factors in OCD patients that might play a fundamental role in the etiopathogenesis of OCD.
■本研究纳入了根据《精神障碍诊断和统计手册》(DSM-V)诊断的39名OCD患者和39名自愿健康受试者。采用荧光法测定血清和红细胞膜中MDA含量。血清TAC水平,血清GSH水平,还使用分光光度法测量了Na/K-ATPase活性。通过反相高效液相色谱法(RP-HPLC)计算血清维生素水平。
■与对照组相比,OCD患者的血清(p<0.0001)和RBC膜(p=0.002)中的MDA水平显着升高。在强迫症患者中发现维生素A(p=0.001)和维生素E(p=0.024)水平显着降低。controls.强迫症患者红细胞膜Na+/K+-ATP酶活性明显低于对照(p<0.0001)。
■我们的发现表明血清和红细胞膜中MDA的水平明显升高,血清维生素A和E水平较低,与对照组相比,OCD患者的膜Na/K-ATPase活性较低。这些表明OCD患者中氧化剂和抗氧化剂因子之间的不平衡,这可能在OCD的病因中起着根本作用。