PDF(Pubmed)
Abstract：
Acne vulgaris represents a chronic inflammatory condition, the pathogenesis of which is closely associated with the altered skin microbiome. Recent studies have implicated a profound role of Gram-negative bacteria in acne development, but there is a lack of antiacne agents targeting these bacteria. Polyphyllins are major components of Rhizoma Paridis with great anti-inflammatory potential. In this study, we aimed to evaluate the antiacne effects and the underlying mechanisms of PPH and a PPH-enriched Rhizoma Paridis extract (RPE) in treating the Gram-negative bacteria-induced acne. PPH and RPE treatments significantly suppressed the mRNA and protein expressions of interleukin (IL)-1β and IL-6 in lipopolysaccharide (LPS)-induced RAW 264.7 and HaCaT cells, along with the intracellular reactive oxygen species (ROS) generation. Furthermore, PPH and RPE inhibited the nuclear translocation of nuclear factor kappa-B (NF-κB) P65 in LPS-induced RAW 264.7 cells. Based on molecular docking, PPH could bind to kelch-like ECH-associated protein 1 (KEAP1) protein. PPH and RPE treatments could activate nuclear factor erythroid 2-related factor 2 (NRF2) and upregulate haem oxygenase-1 (HO-1). Moreover, RPE suppressed the mitogen-activated protein kinase (MAPK) pathway. Therefore, PPH-enriched RPE showed anti-inflammatory and antioxidative effects in vitro, which is promising for alternative antiacne therapeutic.
摘要：
寻常痤疮代表慢性炎症,其发病机制与皮肤微生物组的改变密切相关。最近的研究表明，革兰氏阴性菌在痤疮发展中起着深远的作用，但是缺乏针对这些细菌的抗痤疮剂。Polyphyllins是阴蒂的主要成分，具有很大的抗炎潜力。在这项研究中,我们的目的是评估PPH和富含PPH的重晶石提取物(RPE)治疗革兰氏阴性菌诱导的痤疮的抗痤疮作用和潜在机制.PPH和RPE处理显著抑制脂多糖(LPS)诱导的RAW264.7和HaCaT细胞白细胞介素(IL)-1β和IL-6的mRNA和蛋白表达,随着细胞内活性氧（ROS）的产生。此外，PPH和RPE抑制LPS诱导的RAW264.7细胞中核因子κB（NF-κB）P65的核转位。基于分子对接,PPH可与海带样ECH相关蛋白1（KEAP1）蛋白结合。PPH和RPE处理可以激活核因子红系2相关因子2（NRF2）并上调血红素加氧酶-1（HO-1）。此外，RPE抑制丝裂原活化蛋白激酶(MAPK)途径。因此,富含PPH的RPE在体外显示抗炎和抗氧化作用，这是有希望的替代抗痤疮治疗。
