Abstract：
Bronchoalveolar lavage fluid (BALF) cytology is used for the diagnosis of non-infectious lower airway inflammation in equids. Discrepancies have been reported in the differential cell count when different staining methods were used both in humans and horses. The objective of this study was to compare the results of BALF cytology in donkeys using four different staining methods: modified May-Grunwald Giemsa (mMGG), Diff-Quick (DQ), Toluidine blue (TB) and Perls Prussian blue (PPB). Nine healthy Amiata female donkeys were enrolled. The BAL procedure was performed as previously described and pairs of cytocentrifuged BALF slides were stained with each method. No differences between mMGG and DQ were found for macrophages, neutrophils, and eosinophils, while differences were found in mast cell count between DQ vs.TB, but not between mMGG vs. DQ or mMGG vs. TB. Finally, no differences were obtained in the differential count for hemosiderophages comparing mMGG, DQ and PPB. The mMGG appears to be an excellent stain for the identification of all possible cell types, including mast cells in the BALF of donkeys. DQ, if used alone, may lead to inappropriate identification of mast cells. These results are consistent with the literature on BALF staining methods in horses.
摘要：
支气管肺泡灌洗液（BALF）细胞学检查用于诊断非感染性下气道炎症。当在人和马两者中使用不同的染色方法时,已经报道了差异细胞计数。这项研究的目的是使用四种不同的染色方法比较驴的BALF细胞学结果：改良的May-GrunwaldGiemsa（mMGG），Diff-Quick(DQ),甲苯胺蓝（TB）和Perls普鲁士蓝（PPB）。9只健康的Amiata母驴被招募。如前所述进行BAL程序,并用每种方法对细胞离心的BALF载玻片进行染色。巨噬细胞的mMGG和DQ之间没有差异，中性粒细胞，和嗜酸性粒细胞，而DQ与DQ之间的肥大细胞计数存在差异TB,但不是在mMGG与DQ或mMGG与TB。最后,与mMGG相比，血铁皮噬菌体的分类计数没有差异，DQ和PPB。mMGG似乎是鉴定所有可能的细胞类型的极好的染色剂。包括驴BALF中的肥大细胞.DQ,如果单独使用，可能导致肥大细胞识别不当。这些结果与关于马中BALF染色方法的文献一致。
