Abstract：
Echinococcus granulosus is a zoonotic parasite infects many livestock species, especially cattle, sheep, goat and buffalo, causing cystic echinococcosis. The aim of this study was to demonstrate the presence of the parasite and parasitic tissue damage histopathologically and to determine the role of oxidative stress in the tissue damage through the immunohistochemical detection of the oxidative damage-marker malondialdehyde (MDA) and the antioxidant response-marker superoxide dismutase (SOD). The material of the study consisted of 20 liver samples with Echinococcus cysts and 10 E.granulosus- negative healthy liver samples obtained from different cattle at various times from slaughterhouses in Kırıkkale province, Turkey. Histopathologically, Echinococcus cysts of various sizes were observed along with the surrounding fibrous connective tissue. Giant cells, mononuclear cells, and eosinophilic leukocytes were found between the fibrous connective tissue and the cyst. In the parenchymal tissue distant from the cyst, inflammatory changes were observed, including vacuolation and necrosis in hepatocytes, congestion and dilation sinusoidal capillaries. Immunohistochemically, MDA immunopositivity was observed in both hepatocytes surrounding the cyst and areas distant from the cyst, while SOD immunopositivity was mainly detected in fibrous connective tissue and hepatocytes surrounding the Echinococcus cysts. A significant increase in MDA immunoreactivity was observed in E.granulosus s.l.-infected livers. Although no statistically significant change was observed in SOD immunopositivity in the liver tissues with cystic echinococcosis, regional variations were noted. Germinal layer (GL) of Echinococcus cyst showed immunopositive staining for MDA, while laminated layer (LL) exhibited immunonegative staining. To the authors\' best understanding, this study represents a pioneering effort in showcasing and evaluating the immunoreactivities of MDA and SOD within the liver tissue afflicted with Echinococcus cysts. Simultaneously, the examination extends to encompass tissue damage and the infiltration of inflammatory cells. This study highlights the role of oxidative stress in the pathogenesis of Cystic Echinococcosis (CE) and the need for further investigation of antioxidant defense mechanisms and their regional variations.
摘要：
细粒棘球蚴是一种人畜共患寄生虫，感染许多家畜物种，尤其是牛,绵羊,山羊和水牛,引起囊性包虫病.本研究的目的是通过免疫组织化学检测氧化损伤标记丙二醛（MDA）和抗氧化反应标记超氧化物歧化酶（SOD），从组织病理学上证明寄生虫和寄生虫组织损伤的存在，并确定氧化应激在组织损伤中的作用。该研究的材料包括20个带有棘球蚴囊肿的肝脏样本和10个E.granulosus阴性健康的肝脏样本，这些样本在不同时间从K?r?kkale省的屠宰场获得。土耳其。组织病理学,观察到各种大小的棘球蚴囊肿以及周围的纤维结缔组织。巨细胞,单核细胞,在纤维结缔组织和囊肿之间发现了嗜酸性粒细胞。在远离囊肿的实质组织中，观察到炎症变化，包括肝细胞的空泡化和坏死，阻塞和扩张正弦毛细血管。免疫组织化学，在囊肿周围的肝细胞和远离囊肿的区域观察到MDA免疫阳性。而SOD免疫阳性主要在棘球蚴周围的纤维结缔组织和肝细胞中检测到。在S.I.感染的肝脏中观察到MDA免疫反应性的显着增加。尽管在囊性包虫病的肝组织中没有观察到SOD免疫阳性有统计学意义的变化，注意到区域差异。棘球蚴囊肿的生发层（GL）显示MDA免疫阳性染色，而层压层（LL）表现出免疫阴性染色。对于作者最好的理解，这项研究是一项开创性的工作，旨在展示和评估棘球蚴感染肝组织中MDA和SOD的免疫反应性。同时,检查范围包括组织损伤和炎症细胞浸润。这项研究强调了氧化应激在囊性棘球蚴病（CE）发病机理中的作用，以及进一步研究抗氧化防御机制及其区域变化的必要性。
