Abstract：
A total of 19 resveratrol derivatives, including 12 imines and 7 amines, were synthesized, among which compounds 1, 5, 6, 7\', 11\', and 13 are new compounds. The anti-inflammatory and antitumor activities of these compounds were evaluated in vitro. The results revealed that compounds 1, 6, 8\', 12, and 12\' exhibited significant inhibitory effects (> 50%) on NO production at the concentration of 10 μM and their NO production inhibitory activities have a significant concentration-dependent ability. Additionally, compounds 8\' and 12\' showed promising COX-2 inhibitory activity, and the molecular docking analysis indicated their stable binding to multiple amino acid residues within the active pocket of COX-2 through hydrogen bonding. Moreover, compound 12\' exhibited inhibitory effects on various tumor cell lines and induced apoptosis in MCF-7 breast cancer cells, which was not observed with resveratrol alone. Therefore, the N-substituted structural modification of resveratrol would have possibly enhanced the bioactivity of resveratrol and facilitated its application.
摘要：
共有19种白藜芦醇衍生物，包括12个亚胺和7个胺，是合成的，其中化合物1、5、6、7'，11\'，和13个是新化合物。在体外评价了这些化合物的抗炎和抗肿瘤活性。结果表明，化合物1、6、8、图12和12'在10μM的浓度下对NO产生表现出显著的抑制作用(>50%),并且它们的NO产生抑制活性具有显著的浓度依赖性能力。此外,化合物8和12显示有希望的COX-2抑制活性，分子对接分析表明，它们通过氢键与COX-2活性口袋内的多个氨基酸残基稳定结合。此外，化合物12对各种肿瘤细胞系表现出抑制作用，并诱导MCF-7乳腺癌细胞凋亡，与白藜芦醇单独观察不到。因此,白藜芦醇的N-取代结构修饰可能会增强白藜芦醇的生物活性并促进其应用。
