Abstract：
The increasing global prevalence of antimicrobial resistance in Acinetobacter baumannii has led to concerns regarding the effectiveness of infection treatment. Moreover, the critical role of virulence factor genes in A. baumannii\'s pathogenesis and its propensity to cause severe disease is of particular importance. Comparative genomics, including multi-locus sequence typing (MLST), enhances our understanding of A. baumannii epidemiology. While there is substantial documentation on A. baumannii, a comprehensive study of the antibiotic-resistant mechanisms and the virulence factors contributing to pathogenesis, and their correlation with Sequence Types (STs) remains incompletely elucidated. In this study, we aim to explore the relationship between antimicrobial resistance genes, virulence factor genes, and STs using genomic data from 223 publicly available A. baumannii strains. The core phylogeny analysis revealed five predominant STs in A. baumannii genomes, linked to their geographical sources of isolation. Furthermore, the resistome and virulome of A. baumannii followed an evolutionary pattern consistent with their pan-genome evolution. Among the major STs, we observed significant variations in resistant genes against \"aminoglycoside\" and \"sulphonamide\" antibiotics, highlighting the role of genotypic variations in determining resistance profiles. Furthermore, the presence of virulence factor genes, particularly exotoxin and nutritional / metabolic factor genes, played a crucial role in distinguishing the major STs, suggesting a potential link between genetic makeup and pathogenicity. Understanding these associations can provide valuable insights into A. baumannii\'s virulence potential and clinical outcomes, enabling the development of effective strategies to combat infections caused by this opportunistic pathogen.
摘要：
鲍曼不动杆菌的抗菌素耐药性在全球范围内日益流行，这引起了人们对感染治疗有效性的担忧。此外，毒力因子基因在鲍曼不动杆菌的发病机制及其引起严重疾病的倾向中的关键作用尤其重要。比较基因组学，包括多位点序列分型（MLST），增强了我们对鲍曼不动杆菌流行病学的理解。虽然有关于鲍曼不动杆菌的大量文件，对抗生素耐药机制和致病因素的综合研究，它们与序列类型(ST)的相关性仍未完全阐明。在这项研究中,我们的目的是探讨抗菌素耐药基因之间的关系，毒力因子基因，和STs使用来自223个公开可用的鲍曼不动杆菌菌株的基因组数据。核心系统发育分析揭示了鲍曼不动杆菌基因组中五个主要的STs，与他们孤立的地理来源有关。此外，鲍曼不动杆菌的抗性组和病毒组遵循与其泛基因组进化一致的进化模式。在主要的STS中，我们观察到对“氨基糖苷”和“磺酰胺”抗生素的抗性基因的显着变化，强调基因型变异在确定抗性谱中的作用。此外，毒力因子基因的存在，特别是外毒素和营养/代谢因子基因，在区分主要的STS方面发挥了至关重要的作用，表明基因组成和致病性之间的潜在联系。了解这些关联可以提供有价值的见解鲍曼不动杆菌的毒力潜力和临床结果，能够开发有效的策略来对抗这种机会性病原体引起的感染。
