Abstract：
Toxicodendron vernicifluum bark has been used for many years as a component in foods and as a traditional herbal medication. Unfortunately, the presence of urushiols, which induce allergies, limits its application. This study used a vortex-blending matrix solid-phase dispersion microextraction technique to extract urushiols from Toxicodendron vernicifluum bark. HPLC was used to evaluate the amounts of the extracted urushiols (15:0, 15:1, 15:2, and 15:3). The modified magnetic adsorbent was prepared through an in situ coprecipitation method and characterized using a variety of techniques. The optimized extraction conditions are as follows: using magnetic Zeolite Socony Mobil-Five as an adsorbent, a 1:2 sample/adsorbent ratio, 2.5 min of vortex-blending time, 4 mL of 0.1% (V/V) trifluoroacetic acid-methanol as the elution solvent and 8 min of ultrasound time. There was good linearity and high repeatability in the method. Furthermore, the limits of detection for the urushiols ranged from 0.20 to 0.50 μg/mL. Under the optimized conditions, 50 compounds were identified by ultra high performance liquid chromatography and quadrupole time-of-flight mass spectrometry. These compounds included 8 phenolic acids, 9 monomeric urushiols, 11 urushiol dimers, 10 other components, and 11 flavonoids. The suggested approach, which has the advantages of few stages and high extraction efficiency over existing extraction procedures, is a potentially useful method for obtaining and evaluating urushiols in raw materials or extracts.
摘要：
杜鹃花树皮已被用作食物中的成分和传统的草药药物多年。不幸的是,漆酚的存在，诱发过敏，限制了它的应用。这项研究使用了涡旋混合基质固相分散微萃取技术，从蛇皮树皮中提取了漆酚。HPLC用于评估提取的漆酚的量(15:0、15:1、15:2和15:3)。通过原位共沉淀方法制备了改性的磁性吸附剂，并使用多种技术进行了表征。优化的提取条件如下:以磁性沸石SoconyMobil-Five为吸附剂,a1:2的样品/吸附剂比例，涡流混合时间2.5min,以4mL的0.1%(V/V)三氟乙酸-甲醇为洗脱溶剂,超声时间8min。该方法线性好,重复性高。此外，漆酚的检出限范围为0.20至0.50μg/mL。在优化条件下,通过超高效液相色谱和四极杆飞行时间质谱鉴定了50个化合物。这些化合物包括8种酚酸，9单体漆酚，11漆酚二聚体，其他10个组件和11种类黄酮。建议的方法，与现有提取工艺相比，具有阶段少、提取效率高的优点，是获得和评估原料或提取物中漆酚的潜在有用方法。
