PDF(Pubmed)
Abstract：
Phytophthora blight severely threatens global pepper production. Grafting bolsters plant disease resistance, but the underlying molecular mechanisms remain unclear. In this study, we used P. capsici-resistant strain \'ZCM334\' and susceptible strain \'Early Calwonder\' for grafting. Compared to self-rooted \'Early Calwonder\' plants, \'ZCM334\' grafts exhibited delayed disease onset, elevated resistance, and reduced leaf cell damage, showcasing the potential of grafting in enhancing pepper resistance to P. capsici. Proteomic analysis via the iTRAQ technology unveiled 478 and 349 differentially expressed proteins (DEPs) in the leaves and roots, respectively, between the grafts and self-rooted plants. These DEPs were linked to metabolism and cellular processes, stimulus responses, and catalytic activity and were significantly enriched in the biosynthesis of secondary metabolites, carbon fixation in photosynthetic organizations, and pyruvate metabolism pathways. Twelve DEPs exhibiting consistent expression trends in both leaves and roots, including seven related to P. capsici resistance, were screened. qRT-PCR analysis confirmed a significant correlation between the protein and transcript levels of DEPs after P. capsici inoculation. This study highlights the molecular mechanisms whereby grafting enhances pepper resistance to Phytophthora blight. Identification of key genes provides a foundation for studying the regulatory network governing the resistance of pepper to P. capsici.
摘要：
疫霉疫病严重威胁全球辣椒生产。嫁接支持植物抗病性，但潜在的分子机制仍不清楚。在这项研究中,我们使用抗辣椒菌株“ZCM334”和易感菌株“早期Calwonder”进行嫁接。与自根\'早期Calwonder\'植物相比，\'ZCM334\'移植物表现出延迟的疾病发作，电阻升高，减少叶细胞损伤，展示了嫁接在增强辣椒对辣椒的抗性方面的潜力。通过iTRAQ技术进行的蛋白质组学分析揭示了叶片和根中478和349种差异表达的蛋白质（DEP），分别,在移植物和自根植物之间。这些DEP与新陈代谢和细胞过程有关，刺激反应,和催化活性，并显著富集在次级代谢产物的生物合成中，光合组织中的碳固定，和丙酮酸代谢途径。12个DEP在叶和根表现出一致的表达趋势，包括七个与辣椒芽孢杆菌抗性有关的，被筛选。qRT-PCR分析证实了在接种辣椒后DEP的蛋白质和转录物水平之间的显著相关性。这项研究强调了嫁接增强辣椒对疫霉病的抗性的分子机制。关键基因的鉴定为研究辣椒对辣椒的抗性调控网络奠定了基础。
