PDF(Pubmed)
Abstract：
UNASSIGNED: Antimicrobial resistance (AMR) is becoming a public health concern. Foodborne pathogens are infectious agents that can be transmitted from animals to humans through food and can become resistant due to misuse and overuse of antibiotics, especially in poultry. This study aimed to detect the prevalence of multidrug-resistant and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from local and broiler chickens at the Cibinong market, West Java, Indonesia.
UNASSIGNED: A total of 60 cloacal swab samples from 30 local and broiler chickens sold at the Cibinong market in West Java were obtained by random sampling. From these samples, 39 E. coli isolates were obtained after being cultured on eosin methylene blue agar and molecularly identified using polymerase chain reaction (PCR). Six antibiotic disks were used for the antibiotic sensitivity test against E. coli isolates cultured on Mueller-Hinton agar. PCR was performed to detect ESBL genes (blaTEM, blaSHV, and blaCTX-M).
UNASSIGNED: A total of 76.47% (39/51) cloacal swab samples were positive for E. coli. All E. coli isolates were sensitive to imipenem (100%), and 38 isolates were sensitive to cefoxitin (FOX) (97.4%). On average, the isolates were sensitive to amoxicillin-clavulanic acid (AMC) (69.2%) and ceftriaxone (CRO) (89.7%). E. coli isolates were occasionally resistant to enrofloxacin (25.64%), followed by gentamicin (20.51%), CRO (10.25%), AMC (7.69%), and FOX (2.56%). The prevalence of E. coli AMR was 10.25% (4/39). All four multidrug-resistant E. coli isolates (blaTEM and blaCTX-M) were confirmed to have the ESBL gene based on PCR.
UNASSIGNED: The prevalence of multidrug-resistant and ESBL-producing E. coli is still found, proving that there is still inappropriate use of antibiotics and a need for strict supervision of their use, especially around Cibinong market, West Java.
UNASSIGNED: A total of 60 cloacal swab samples from 30 local and broiler chickens sold at the Cibinong market in West Java were obtained by random sampling. From these samples, 39 E. coli isolates were obtained after being cultured on eosin methylene blue agar and molecularly identified using polymerase chain reaction (PCR). Six antibiotic disks were used for the antibiotic sensitivity test against E. coli isolates cultured on Mueller-Hinton agar. PCR was performed to detect ESBL genes (blaTEM, blaSHV, and blaCTX-M).
UNASSIGNED: A total of 76.47% (39/51) cloacal swab samples were positive for E. coli. All E. coli isolates were sensitive to imipenem (100%), and 38 isolates were sensitive to cefoxitin (FOX) (97.4%). On average, the isolates were sensitive to amoxicillin-clavulanic acid (AMC) (69.2%) and ceftriaxone (CRO) (89.7%). E. coli isolates were occasionally resistant to enrofloxacin (25.64%), followed by gentamicin (20.51%), CRO (10.25%), AMC (7.69%), and FOX (2.56%). The prevalence of E. coli AMR was 10.25% (4/39). All four multidrug-resistant E. coli isolates (blaTEM and blaCTX-M) were confirmed to have the ESBL gene based on PCR.
UNASSIGNED: The prevalence of multidrug-resistant and ESBL-producing E. coli is still found, proving that there is still inappropriate use of antibiotics and a need for strict supervision of their use, especially around Cibinong market, West Java.
摘要：
■抗菌素耐药性（AMR）正在成为公共卫生问题。食源性病原体是可以通过食物从动物传播给人类的传染性病原体，并且由于滥用和过度使用抗生素而产生抗药性，尤其是家禽。这项研究旨在检测从慈比农市场的本地和肉鸡中分离出的多药耐药和产超广谱β-内酰胺酶（ESBL）的大肠杆菌的流行情况，西爪哇,印度尼西亚。
■通过随机抽样，从西爪哇Cibinong市场出售的30只本地和肉鸡中获得了60只泄殖腔拭子样本。从这些样本中,在伊红亚甲基蓝琼脂上培养后获得39株大肠杆菌，并使用聚合酶链反应（PCR）进行分子鉴定。六个抗生素盘用于针对在Mueller-Hinton琼脂上培养的大肠杆菌分离株的抗生素敏感性测试。进行PCR以检测ESBL基因(blaTEM，blaSHV,和blaCTX-M）。
■总共76.47%(39/51)的泄殖腔拭子样本对大肠杆菌呈阳性。所有大肠杆菌分离株对亚胺培南(100%)敏感,38株对头孢西丁(FOX)敏感(97.4%)。平均而言,分离株对阿莫西林克拉维酸(AMC)(69.2%)和头孢曲松(CRO)(89.7%)敏感。大肠杆菌分离株偶尔对恩诺沙星耐药(25.64%)，其次是庆大霉素(20.51%)，CRO（10.25%），AMC（7.69%），和福克斯（2.56%）。大肠杆菌AMR的患病率为10.25%(4/39)。基于PCR确认所有四种多重耐药大肠杆菌分离株(blaTEM和blaCTX-M)具有ESBL基因。
■仍然发现多药耐药和产生ESBL的大肠杆菌的流行，证明仍然存在抗生素的不当使用，并且需要对其使用进行严格的监督，特别是在慈宾农市场，西爪哇。
■通过随机抽样，从西爪哇Cibinong市场出售的30只本地和肉鸡中获得了60只泄殖腔拭子样本。从这些样本中,在伊红亚甲基蓝琼脂上培养后获得39株大肠杆菌，并使用聚合酶链反应（PCR）进行分子鉴定。六个抗生素盘用于针对在Mueller-Hinton琼脂上培养的大肠杆菌分离株的抗生素敏感性测试。进行PCR以检测ESBL基因(blaTEM，blaSHV,和blaCTX-M）。
■总共76.47%(39/51)的泄殖腔拭子样本对大肠杆菌呈阳性。所有大肠杆菌分离株对亚胺培南(100%)敏感,38株对头孢西丁(FOX)敏感(97.4%)。平均而言,分离株对阿莫西林克拉维酸(AMC)(69.2%)和头孢曲松(CRO)(89.7%)敏感。大肠杆菌分离株偶尔对恩诺沙星耐药(25.64%)，其次是庆大霉素(20.51%)，CRO（10.25%），AMC（7.69%），和福克斯（2.56%）。大肠杆菌AMR的患病率为10.25%(4/39)。基于PCR确认所有四种多重耐药大肠杆菌分离株(blaTEM和blaCTX-M)具有ESBL基因。
■仍然发现多药耐药和产生ESBL的大肠杆菌的流行，证明仍然存在抗生素的不当使用，并且需要对其使用进行严格的监督，特别是在慈宾农市场，西爪哇。
