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Abstract：
Background In patients with rheumatoid arthritis, oxidative DNA damage is increased by deficient zinc levels as well as increasing disease activity. However, the relationship between zinc levels, disease activity, and oxidative DNA damage remains unclear. In this study, we investigated serum zinc levels and disease activity and their association with 8-hydroxy-2-deoxyguanosine (8-OHdG). Methodology This case-control study was conducted among rheumatoid arthritis patients (n = 264) and healthy individuals (n = 192). Oxidative DNA damage was assessed by measuring serum 8-OHdG using enzyme-linked immunosorbent assay. Colorimetry was used to measure serum zinc levels. Disease activity was assessed using the Disease Activity Score-28 (DAS-28) score. Results Significantly higher 8-OHdG levels (p < 0.00) were found in the test group compared to the control group. Moreover, significantly lower serum zinc levels (p < 0.001) were noted in patients with rheumatoid arthritis compared to the control group. In addition, higher 8-OHdG levels were found in patients with low serum zinc levels compared to those with normal mean serum zinc levels. Lower levels of DNA oxidative damage were found in patients with moderate and low disease activity compared to those with high disease activity. A significant negative correlation was noted between serum zinc levels and DAS-28 scores and oxidative DNA damage marker (r = - 0.30, p = 0.038 and r = - 0.26, p = 0.043, respectively), while a significant positive correlation was observed between body mass index and 8-OHdG (r = 0.22, p = 0.02) in healthy individuals. Conclusions High serum 8-OHdG levels and high disease activity with low mean serum zinc levels may indicate a high degree of oxidative DNA damage in patients with rheumatoid arthritis.
摘要：
背景在类风湿关节炎患者中，锌水平缺乏以及疾病活动增加会增加氧化DNA损伤。然而,锌水平之间的关系，疾病活动,氧化性DNA损伤仍不清楚。在这项研究中,我们调查了血清锌水平和疾病活动性,以及它们与8-羟基-2-脱氧鸟苷(8-OHdG)的关系.方法本病例对照研究在类风湿关节炎患者（n=264）和健康个体（n=192）中进行。通过使用酶联免疫吸附测定法测量血清8-OHdG来评估氧化性DNA损伤。比色法用于测量血清锌水平。使用疾病活动评分-28(DAS-28)评分评估疾病活动。结果与对照组相比,在试验组中发现显著更高的8-OHdG水平(p<0.00)。此外，与对照组相比,类风湿关节炎患者血清锌水平显著降低(p<0.001).此外,与平均血清锌水平正常的患者相比,血清锌水平低的患者的8-OHdG水平更高.与疾病活动性高的患者相比，中度和低疾病活动性的患者的DNA氧化损伤水平较低。血清锌水平与DAS-28评分和氧化DNA损伤标志物之间存在显著负相关(分别为r=-0.30,p=0.038和r=-0.26,p=0.043),在健康个体中，体重指数与8-OHdG之间存在显着正相关（r=0.22，p=0.02）。结论高血清8-OHdG水平和高疾病活动度和低平均血清锌水平可能表明类风湿关节炎患者存在高度的DNA氧化损伤。
