Abstract：
Soybean proteins (pro) and soybean peptides (pep) are beneficial to the growth and metabolism of Limosilactobacillus reuteri (L. reuteri). However, whether they could assist L. reuteri in inhibiting intestinal pathogens and the inhibition mode of them is still unclear. In this study, a co-culture experiment of L. reuteri LR08 with Escherichia coli JCM 1649 (E. coli) was performed. It showed that pro and pep could still favour the growth of L. reuteri over E. coli under their competition. The inhibition zone experiment showed the digested soybean proteins (dpro) could improve its antibacterial activity by increasing the secretion of organic acids from L. reuteri. Furthermore, digested soybean peptides (dpep) could enhance nitrogen utilization capacity of L. reuteri over E. coli. These results explained the patterns of dpro and dpep assisting L. reuteri in inhibiting the growth of E. coli by regulating its organic acid secretion and the ability of nitrogen utilization.
摘要：
大豆蛋白（pro）和大豆肽（pep）有利于罗伊利氏杆菌的生长和代谢（L.reuteri）。然而,它们是否能帮助罗伊氏乳杆菌抑制肠道病原体及其抑制方式尚不清楚。在这项研究中,罗伊氏乳杆菌LR08与大肠杆菌JCM1649的共培养实验(E.大肠杆菌)进行。结果表明，在竞争下，pro和pep仍然可以比大肠杆菌更有利于罗伊氏乳杆菌的生长。抑制区实验表明，消化的大豆蛋白（dpro）可以通过增加罗伊乳杆菌有机酸的分泌来提高其抗菌活性。此外，消化大豆肽（dpep）可以提高罗伊乳杆菌对大肠杆菌的氮利用能力。这些结果解释了dpro和dpep通过调节大肠杆菌的有机酸分泌和氮利用能力来帮助罗伊氏乳杆菌抑制大肠杆菌生长的模式。
