Abstract：
Nanoparticle-based delivery systems have emerged as powerful tools in the field of pest management, offering precise and effective means of delivering double-stranded RNA (dsRNA), a potent agent for pest control through RNA interference (RNAi). This comprehensive review aims to evaluate and compare various types of nanoparticles for their suitability in dsRNA delivery for pest management applications. The review begins by examining the unique properties and advantages of different nanoparticle materials, including clay, chitosan, liposomes, carbon, gold and silica. Each material\'s ability to protect dsRNA from degradation and its potential for targeted delivery to pests are assessed. Furthermore, this review delves into the surface modification strategies employed to enhance dsRNA delivery efficiency. Functionalization with oligonucleotides, lipids, polymers, proteins and peptides is discussed in detail, highlighting their role in improving stability, cellular uptake, and specificity of dsRNA delivery.This review also provides valuable guidance on choosing the most suitable nanoparticle-based system for delivering dsRNA effectively and sustainably in pest management. Moreover, it identifies existing knowledge gaps and proposes potential research directions aimed at enhancing pest control strategies through the utilization of nanoparticles and dsRNA.
摘要：
基于纳米颗粒的递送系统已经成为害虫管理领域的强大工具，提供精确有效的双链RNA（dsRNA）传递方式，通过RNA干扰（RNAi）控制害虫的有效试剂。这篇综合综述旨在评估和比较各种类型的纳米颗粒在dsRNA递送中的适用性，以用于害虫管理应用。该综述首先考察了不同纳米颗粒材料的独特性能和优势，包括粘土,壳聚糖,脂质体，碳,金和二氧化硅。评估每种材料保护dsRNA免于降解的能力及其靶向递送至害虫的潜力。此外，这篇综述深入研究了用于增强dsRNA递送效率的表面修饰策略。用寡核苷酸功能化，脂质，聚合物，详细讨论了蛋白质和肽，强调它们在提高稳定性方面的作用，细胞摄取，和dsRNA递送的特异性。这篇综述还为选择最合适的基于纳米颗粒的系统在害虫管理中有效和可持续地递送dsRNA提供了有价值的指导。此外，它确定了现有的知识差距，并提出了潜在的研究方向，旨在通过利用纳米颗粒和dsRNA来加强害虫控制策略。
