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Abstract：
BACKGROUND: The purpose of this study was to assess the antifungal activity of silver nanoparticles (AgNPs) in combination with calcium hydroxide (Ca(OH)2) against Candida albicans (C. albicans).
METHODS: AgNPs was mixed with pure Ca(OH)2 powder in an aqueous base. A standard suspension (1 × 108 bacterial cells/mL) of C. albicans was prepared in a 96-well plate and incubated on shaker at 37 °C in 100% humidity to allow fungal biofilm formation in infected dentin slices (n = 98). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AgNPs alone or with Ca(OH)2 were determined. The samples were separately placed in 24-well tissue culture plates and divided into three experimental groups (0.03, 0.04, and 0.06) and three control groups; negative (saline) and positive chlorhexidine gel and Ca(OH)2. Quantitative measurements of fungal activity by XTT colorimetric assay and qualitative measurements using confocal laser microscopy and scanning electron microscopy were performed.
RESULTS: The cell viability of C. albicans in the experimental groups was significantly reduced compared to the negative control group. The combination of (AgNPs (0.04%) and Ca(OH)2) was the most potent against C. albicans.
CONCLUSIONS: The findings demonstrated that combining silver nanoparticles with Ca(OH)2 was more effective against C. albicans biofilm compared to Ca(OH)2 alone, suggesting a combing effect.
METHODS: AgNPs was mixed with pure Ca(OH)2 powder in an aqueous base. A standard suspension (1 × 108 bacterial cells/mL) of C. albicans was prepared in a 96-well plate and incubated on shaker at 37 °C in 100% humidity to allow fungal biofilm formation in infected dentin slices (n = 98). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AgNPs alone or with Ca(OH)2 were determined. The samples were separately placed in 24-well tissue culture plates and divided into three experimental groups (0.03, 0.04, and 0.06) and three control groups; negative (saline) and positive chlorhexidine gel and Ca(OH)2. Quantitative measurements of fungal activity by XTT colorimetric assay and qualitative measurements using confocal laser microscopy and scanning electron microscopy were performed.
RESULTS: The cell viability of C. albicans in the experimental groups was significantly reduced compared to the negative control group. The combination of (AgNPs (0.04%) and Ca(OH)2) was the most potent against C. albicans.
CONCLUSIONS: The findings demonstrated that combining silver nanoparticles with Ca(OH)2 was more effective against C. albicans biofilm compared to Ca(OH)2 alone, suggesting a combing effect.
摘要：
背景：本研究的目的是评估银纳米颗粒（AgNPs）与氢氧化钙（Ca（OH）2）组合对白色念珠菌的抗真菌活性（C.albicans).
方法:将AgNP与纯Ca(OH)2粉末在含水基质中混合。在96孔板中制备白色念珠菌的标准悬浮液(1×108个细菌细胞/mL),并在37℃下在100%湿度下在振荡器上孵育,以允许在感染的牙本质切片中形成真菌生物膜(n=98)。测定单独或与Ca(OH)2-起的AgNPs的最小抑制浓度(MIC)和最小杀菌浓度(MFC)。将样品分别置于24孔组织培养板中，并分为三个实验组（0.03、0.04和0.06）和三个对照组；阴性（盐水）和阳性氯己定凝胶和Ca（OH）2。通过XTT比色法定量测量真菌活性，并使用共聚焦激光显微镜和扫描电子显微镜进行定性测量。
结果：与阴性对照组相比，实验组中白色念珠菌的细胞活力显着降低。(AgNP(0.04%)和Ca(OH)2)的组合对白色念珠菌是最有效的。
结论：研究结果表明，与单独使用Ca（OH）2相比，将银纳米颗粒与Ca（OH）2结合对白色念珠菌生物膜更有效，暗示了梳理效果。
方法:将AgNP与纯Ca(OH)2粉末在含水基质中混合。在96孔板中制备白色念珠菌的标准悬浮液(1×108个细菌细胞/mL),并在37℃下在100%湿度下在振荡器上孵育,以允许在感染的牙本质切片中形成真菌生物膜(n=98)。测定单独或与Ca(OH)2-起的AgNPs的最小抑制浓度(MIC)和最小杀菌浓度(MFC)。将样品分别置于24孔组织培养板中，并分为三个实验组（0.03、0.04和0.06）和三个对照组；阴性（盐水）和阳性氯己定凝胶和Ca（OH）2。通过XTT比色法定量测量真菌活性，并使用共聚焦激光显微镜和扫描电子显微镜进行定性测量。
结果：与阴性对照组相比，实验组中白色念珠菌的细胞活力显着降低。(AgNP(0.04%)和Ca(OH)2)的组合对白色念珠菌是最有效的。
结论：研究结果表明，与单独使用Ca（OH）2相比，将银纳米颗粒与Ca（OH）2结合对白色念珠菌生物膜更有效，暗示了梳理效果。
